Figure 7From: A mechanistic basis for amplification differences between samples and between genome regionsLigase treatment drastically reduces PCR efficiency in TUF regions. The charts indicate the amplicon product ratios for two PRT assays for four ‘old’ DNA samples (C7, C8, D4, D5) that were untreated (blue) and treated with PreCR (red), which includes a ligase to repair ssDNA nicks. Treated samples have greatly reduced amplification efficiency at the test amplicon in the ‘TUF’ region compared to the untreated DNA samples.Back to article page