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Table 2 List of over-expressed proteases and antiproteases in livers of laying hens

From: Transcriptomic profiling of proteases and antiproteases in the liver of sexually mature hens in relation to vitellogenesis

Protein name [Gallus gallus]

GeneID/Ensembl transcript

Ratio ML / IML

Superfamily/domains

Activity

  

Oligoarray

q-RT-PCR

  

PREDICTED: cathepsin E-A-like/similar to nothepsin

417848/ ENSGALT00000016271

34.8

31.1b

Peptidase A1

Aspartate protease

PREDICTED: uncharacterized protein LOC419301/similar to porin

419301/ ENSGALT00000011045

20.9

68.9b

Kunitz/WAP

Serine protease inhibitor

PREDICTED: ovochymase-2/similar to oviductin

769290/ ENSGALT00000008522

4.0

6.2a

Peptidase S1

Serine protease

PREDICTED: papilin

428873/ ENSGALT00000015156

2.7

3.9b

Kunitz

Serine protease inhibitor

PREDICTED: ubiquitin carboxyl-terminal hydrolase 3

415369/ ENSGALT00000005420

2.7

2.3b

Peptidase C19

Ubiquitinyl hydrolase

PREDICTED: aminopeptidase O

427467/ ENSGALT00000020596

2.1

1.8a

Peptidase M1

Aminopeptidase

PREDICTED: A disintegrin and metalloproteinase with thrombospondin motifs 17

415515/ ENSGALT00000011448

2.1

2.5a

Peptidase M12B

Zinc metallopeptidase

PREDICTED: proprotein convertase subtilisin/kexin type 6

395454/ ENSGALT00000011615

2.0

2.9b

Peptidase S8

Serine protease

PREDICTED: glutamyl aminopeptidase

428771/ ENSGALT00000014775

1.9

3.1a

Peptidase M28

Glutamate carboxypeptidase II-like

PREDICTED: N-acetylated alpha-linked acidic dipeptidase-like 2

429156/ENSGALT00000019779

1.9

1.7b

Peptidase M1

Aminopeptidase

PREDICTED: A disintegrin and metalloproteinase with thrombospondin motifs 5

427971/ ENSGALT00000025470

1.7

1.6NS

Peptidase M12B

Zinc metalloprotease

PREDICTED: chymotrypsin inhibitor-like

768734/ ENSGALT00000035386

1.7

1.6a

Kazal

Serine protease inhibitor

PREDICTED: OTU domain-containing protein 3

426746/ ENSGALT00000022924

1.6

1.9b

Peptidase C65

Cysteine protease

PREDICTED: TRAF3-interacting protein 1

424029/ ENSGALT00000006731

1.5

1.6b

Peptidase C19

Ubiquitinyl hydrolase

PREDICTED: metalloendopeptidase OMA1, mitochondrial

424670/ENSGALT00000017659

1.4

1.2NS

Peptidase M48

Zinc Metalloprotease

PREDICTED: mitochondrial inner membrane protease subunit 2 isoform 2

417780/ ENSGALT00000015446

1.4

2.1b

Peptidase S24/S26

LexA-related/ type I signal peptidase

PREDICTED: PPPDE peptidase domain- containing protein 2-like

770448/ ENSGALT00000019464

1.4

1.5b

DUF862/ PPPDE

Predicted thiol peptidase

Signal peptide peptidase-like 2A precursor

415450/ ENSGALT00000038799

1.3

0.9NS

Peptidase A22B

Signal peptide peptidase

  1. For oligoarray analysis, statistical significance was determined as described in Methods. For Q-RT-PCR analysis, statistical significance was measured at 5% level (P < 0.05, a) and whereas highly statistical significance was measured at 1% level (P < 0.01, b). Non-significant results were denoted by NS (P > 0.05). Protein names were collected from NCBI sequence annotation that was available on 02-15-2012 (http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/protein). ML: livers of sexually mature hens; IML, livers of pre-laying pullets (immature pullets).