Transcriptomic profiling of proteases and antiproteases in the liver of sexually mature hens in relation to vitellogenesis

Table 4 Proteases identified in the egg yolk [[14],[15]] and the vitelline membrane (VM) [[30]]

Proteases name [*Gallus gallus*]	GeneID/Ensembl Transcript ID	Yolk proteome [[14],[15]]	Vitelline membrane proteome [[30]]	Oligoarray ID	Ratio ML/IML (Transcriptome)
56 kDa protein	428163/ENSGALT00000011138	+		RIGG00309	0.691
Aminopeptidase N (Ey)	395667/ENSGALT00000013470	+		RIGG14412	0.929
Anticoagulant protein C	395085/ENSGALT00000002745	+		RIGG07767	1.084
Coagulation factor IX	374258/ENSGALT00000010525	+		RIGG13402	1.020
Coagulation factor X	415267/ENSGALT00000027175	+		Missing
Complement factor B-like protease	- ^(b)	+		?
Hepatocyte growth factor like	396135/ENSGALT00000036285	+		RIGG07902	0.829
PREDICTED: cathepsin E-A-like/nothepsin	417848/ENSGALT00000016271	+		RIGG15447	34.846
Similar to carboxypeptidase D	417590/ENSGALT00000006838	+		RIGG12160	0.916
Similar to complement component C2	419574/ENSGALT00000041167	+		Missing
Similar to Hepatocyte growth factor activator	422876/ENSGALT00000025185	+		RIGG09473	1.169
PREDICTED:Ovochymase-2/similar to oviductin	769290/ENSGALT00000008522		+	RIGG08201	4.014
Similar to plasminogen	421580/ENSGALT00000006829	+		RIGG08074	0.896
Similar to thrombin-activable fibrinolysis inhibitor	418851/ENSGALT00000027441	+		RIGG09638	1.018
Similar to transmembrane protease serine 13	419770/ENSGALT00000011876	+		RIGG05703	-^(a)
Similar to transmembrane protease serine 9	428320/ENSGALT00000000620	+	+	RIGG07595	-^(a)
Similar to ubiquitin specific protease 42	416431/ENSGALT00000005338	+		RIGG11623	-^(a)
Thrombin	395306/ENSGALT00000013559	+		RIGG14442	0.978

ML: livers of sexually mature hens; IML, livers of pre-laying pullets (immature pullets).
^(a)There was no statistical evidence of the expression of these genes in our transcriptomic approach. ^(b) No ensembl transcript or gene could be identified for this protein (ID IPI00588708) as reported in [2].
Proteases which were identified in the viltelline membrane and/or the egg yolk at a proteomic level but which were not spotted on the oligoarray are indicated in italics.

ISSN: 1471-2164