Figure 7From: Mining and characterization of ubiquitin E3 ligases expressed in the mouse testisLigase activity of putative E3s was validated by in vivo assays. (A) HEK 293FT cells were transfected together with FLAG-E3s and HA-Ub. After MG-132 treatment, whole cell lysate was subjected to immunoprecipitation with anti-FLAG beads. Poly-ubiquitination was detected by Western blotting with anti-FLAG and anti-HA antibody. (B) Protein mixture was subjected to anti-FLAG beads after heating with 1% SDS.Back to article page