Figure 2

Southern blot analysis of DNA from the MugugaUganda recombinant clone and parental Muguga and Uganda clones using a telomeric DNA probe. A blot of Eco RI-digested T. parva DNA that was size fractionated through a 0.8% agarose gel and transferred onto a Hybond N Membrane is depicted. The blot was hybridised with a telomeric probe [48] and washed in 2X SSC at 60° C for 1 hour. The lanes are (1) T. parva Muguga cloned piroplasm DNA (1 ug), (2) T. parva Uganda schizont-infected lymphocyte DNA (20ug), (3) T. parva Uganda schizont-infected lymphocyte DNA (1ug) and (4) T. parva MugugaUganda recombinant clone piroplasm DNA (1ug). On the right hand side of the blot, the letter ‘A’ indicates two size polymorphic Eco RI restriction fragments of presumptive T. parva Muguga parental origin and the letter ‘B’ indicates three fragments of presumptive T. parva Uganda parental origin in the recombinant clone. Three telomeric fragments of T. parva Muguga parental origin that are absent from the recombinant parasite clone are indicated by an arrow on the left hand side of the blot, highlighted by the letter M. One telomeric fragment of T. parva Uganda origin is also inidicated by an arrow with the legend UG. Large Eco RI fragments that were not polymorphic using size fractionation through 0.8% agarose are indicated by an arrow and the letter UN on the left hand side of the blot. There were no detectable fragments that were clearly unique to the recombinant parasite.