Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway

Fisher, Katherine H; Wright, Victoria M; Taylor, Amy; Zeidler, Martin P; Brown, Stephen

doi:10.1186/1471-2164-13-506

BMC Genomics

Table 3 Off-target analysis of HFA and SRSF hit lists

From: Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway

On target			Predicted off target
On target - dsRNA	On target - Gene	On target - score	Off target – Flybase ID	Off target - Gene	Off target - dsRNA	Off target - score
HFA16984	larp	-3.1	FBgn0003013	*osa*	HFA17022	-4.2	*
HFA18710	RpS14a	-6.1	FBgn0004404	*RpS14b*	HFA18711	-4.9	*
HFA19901	CG4411	-2.3	FBgn0039633	CG11873	BKN26700	2.2
			FBgn0028991	*CG4055*	HFA06863	-3.7	*
			FBgn0043884	*mask*	HFA16018	-5.6	*
			FBgn0043884	*mask*	HFA16005	-5.8	*
			FBgn0043884	*mask*	BKN20625	-4.3	*
			FBgn0004656	*fs(1)h*	HFA18778	-4.1	*
			FBgn0086902	kis	BKN20986	2.3
			FBgn0004864	*hop*	HFA20340	-5.8	*
			FBgn0004864	*hop*	BKN24272	-5.7	*
HFA12365	Rm62	3.1	FBgn0035720	*CG10077*	HFA09691	3.1	*
HFA07091	CG8179	4.9	FBgn0020306	dom	BKN21379	-3.4
			FBgn0015618	Cdk8	HFA11113	-2.3
			FBgn0015903	*apt*	HFA04671	3.2	*
			FBgn0026575	CG4411	HFA19901	-2.1
			FBgn0260794	ctrip	BKN20611	-2.6
			FBgn0028991	CG4055	HFA06863	-3.7
			FBgn0039633	*CG11873*	BKN26700	2.2	*
			FBgn0000008	CG14169	HFA10170	-2.2
			FBgn0003013	osa	HFA17022	-4.2
			FBgn0051716	*Cnot4*	BKN22063	2.1	*
			FBgn0260724	larp	HFA16984	-2.9
			FBgn0035720	*CG10077*	HFA09691	3.1	*
			FBgn0011666	msi	HFA17003	-2.6
			FBgn0003507	*srp*	HFA17068	13.6	*
			FBgn0003507	*srp*	BKN45799	8.6	*
			FBgn0004656	fs(1)h	HFA18778	-4.1
			FBgn0003687	Tbp	BKN20836	-4.5
			FBgn0012049	msi	HFA17003	-2.6
			FBgn0004606	*zfh1*	BKN29931	5.2	*
			FBgn0001078	*ftz-f1*	BKN28995	2.3	*
			FBgn0086902	*kis*	BKN20986	2.3	*
			FBgn0034258	CG4954	HFA06905	-5.5
			FBgn0027492	CkIIbeta	HFA20230	-2.2
			FBgn0032633	CG4055	HFA06863	-3.7
			FBgn0043884	mask	HFA16018	-5.6
			FBgn0043884	CG6268	HFA16005	-5.8
			FBgn0043884	mask	BKN20625	-4.3
HFA06863	CG4055	-4.2	FBgn0015618	*Cdk8*	HFA11113	-2.3	*
			FBgn0000008	*CG14169*	HFA10170	-2.2	*
			FBgn0003507	srp	HFA17068	13.6
			FBgn0003507	srp	BKN45799	8.6
			FBgn0039633	CG11873	BKN26700	2.2
			FBgn0001078	ftz-f1	BKN28995	2.3
			FBgn0086902	kis	BKN20986	2.3
HFA04671	apt	2.7	FBgn0001078	*ftz-f1*	BKN28995	2.3	*
			FBgn0030636	cngl	HFA19089	-2.7
			FBgn0003507	*srp*	HFA17068	13.6	*
			FBgn0003507	*srp*	BKN45799	8.6	*
HFA18711	RpS14b	-6.0	FBgn0004403	*RpS14a*	HFA18710	-4.9	*
HFA17068	srp	8.5	FBgn0030636	cngl	HFA19089	-2.7
			FBgn0020306	dom	BKN21379	-3.4
			FBgn0015618	Cdk8	HFA11113	-2.3
			FBgn0039633	*CG11873*	BKN26700	2.2	*
			FBgn0260794	ctrip	BKN20611	-2.6
			FBgn0003016	*kis*	BKN20986	2.3	*
			FBgn0086758	*CG17156*	HFA00485	7.0	*
			FBgn0086758	*CG17649*	HFA00509	7.0	*
			FBgn0086758	*chinmo*	BKN45751	6.6	*
			FBgn0005630	*lola*	BKN30256	3.1	*
			FBgn0000259	CkIIbeta	HFA20230	-2.2
			FBgn0028371	CG13525	HFA04167	-2.9
			FBgn0032633	CG4055	HFA06863	-3.7
			FBgn0015903	*apt*	HFA04671	3.2	*
			FBgn0026575	CG4411	HFA19901	-2.1
			FBgn0001078	*ftz-f1*	BKN28995	2.3	*
			FBgn0011666	msi	HFA17003	-2.6
			FBgn0028991	CG4055	HFA06863	-3.7
			FBgn0035720	*CG10077*	HFA09691	3.1	*
			FBgn0000008	CG14169	HFA10170	-2.2
			FBgn0086902	*kis*	BKN20986	2.3	*
HFA09691	CG10077	2.6	FBgn0003261	*Rm62*	HFA12365	3.8	*
BKN41059	CG40121	-3.6	FBgn0043903	*dome*	BKN25660	-7.9	*
			FBgn0000259	*CkIIbeta*	HFA20230	-2.2	*
			FBgn0004656	*fs(1)h*	HFA18778	-4.8	*
			FBgn0043903	*dome*	HFA19583	-13.5	*
			FBgn0010412	*RpS19*	HFA20281	-7.0	*

Bold and * = labelling of off-targets indicates that their knockdown resulted in a significant Z-score in the same direction as the original intended gene.
On-target dsRNAs with potentially interacting off-targets identified in each screen.

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