Large-scale sequencing based on full-length-enriched cDNA libraries in pigs: contribution to annotation of the pig genome draft sequence

Table 4 Distributions of lengths of cDNA clones completely sequenced by the primer walking and transposon shotgun sequencing methods

Length (bp)	Sequenced using universal primersa	Primer walking			Transposon shotgun sequencing
Length (bp)	Sequenced using universal primersa	Once	Twice	Total	Transposon shotgun sequencing
–1000	1174	1956	93	2049	156
1001–1500	928	9062	913	9975	519
1501–2000	67	6201	1843	8044	1058
2001–2500	0	2817	731	3548	766
2501–3000	0	919	428	1347	450
3000–3500	0	48	294	342	286
3501–4000	0	1	85	86	165
4001–	0	0	19	19	100
Total	2169	21,004	4406	25,410	3500
Average (bp)	907.6	1548.3	1959.6	1620.0	2163.3

Lengths of cDNA clones are shown according to the sequencing methods used. Clones sequenced by using the primer walking (PW) method are shown separately according to the number of primer walkings. Clones sequenced by using transposon shotgun sequencing (TPS) include those that were finished by the TPS method after being sequenced by the PW method.
^a Clones that were completely sequenced just by using universal primers (forward and reverse primers aligned to the vector sequence, and T₂₅V primer).

ISSN: 1471-2164