Skip to main content

Table 4 Transcript abundance of each GH11, GH28 and GH45 in P . cochleariae larvae and adults based on a mapping of Illumina reads to the ORFs of the PCWDEs

From: Combining proteomics and transcriptome sequencing to identify active plant-cell-wall-degrading enzymes in a leaf beetle

Name

cds (bp)a

LGb

LRb

AGb

ARb

 

Readsc

 

RPKMd

readsc

RPKMd

readsc

RPKMd

readsc

RPKMd

GH11

GH11-2*

654

7091

14,46935

18

7,59882

4098

13,81739

2

4,09316

GH11-1*

654

4018

13,64994

13

7,12934

2375

13,03126

1

3,09316

GH28

GH28-9*

1113

104975

17,62099

420

11,3895

108541

17,8029

69

8,51589

GH28-1*

1101

52323

16,60188

182

10,18524

33084

16,07982

16

6,3417

GH28-3*

1116

25617

15,55142

121

9,58866

23003

15,53571

39

7,60758

GH28-4

1110

17208

14,98799

42

8,05802

14477

14,87829

54

8,08485

GH28-2

1089

10944

14,36062

64

8,69326

12377

14,67758

31

7,31171

GH28-7*

1125

10075

14,19475

33

7,69073

20121

15,33691

19

6,55852

GH28-6*

1128

5395

13,28797

18

6,81242

8822

14,13731

10

5,62868

GH28-8

1125

2254

12,03587

18

6,81626

2093

12,07132

14

6,11795

GH28-5

1110

2213

12,02813

30

7,5758

2618

12,40896

23

6,85352

GH45

GH45-1*

729

25102

16,13723

104

9,97271

21358

16,04375

15

6,84342

GH45-3*

732

15902

15,51018

102

9,96678

13460

15,39577

33

8,05989

GH45-8

729

15813

15,47135

73

9,46209

17763

15,77897

21

7,32885

GH45-7*

726

13114

15,20774

60

9,18511

15761

15,61229

24

7,52744

GH45-4*

723

6088

14,11546

21

7,67651

10930

15,09838

8

6,11838

GH45-5*

723

4651

13,72137

27

8,03908

6289

14,2971

22

7,40788

GH45-6

726

365

10,04911

1

3,27822

557

10,79833

12

6,52744

References

eIF4A

1263

11457

14,21229

14111

16,26465

10960

14,28746

11633

15,64997

EF1α

1389

67267

16,62849

90280

18,80497

63998

16,69593

61492

17,91526

  1. aLength of the full length coding DNA sequences (cds) in base pairs. bSequences were generated from four independent RNA pools: larval gut (LG), larval rest body (LR), adult gut (AG) and adult rest body (AR). cAbsolute numbers of reads mapped to each ORF corresponding to a putative PCWDE for each specific RNA pool. dThe number of reads per kilobase and per million (RPKM values) are log2 transformed (see ‘Experimental section’). * indicates that the corresponding proteins were identified from the proteomic analysis.