Table 2 Characteristics of 94 polymorphic SSR markers developed in Vicia faba L. (F=forward primer, R=reverse primer, Size = size of cloned allele, Ta = annealing temperature)
From: High-throughput novel microsatellite marker of faba bean via next generation sequencing
Primer | Repeat | F (5’– 3’) | R (5’– 3’) | Size (bp) | Ta(°C) |
|---|---|---|---|---|---|
CAAS1 | (AAAGGG)7 | AGTCAGGGGGTCGATTTTTC | TCTTGCGCAGTTTTGACATC | 212 | 55 |
CAAS2 | (GAA)9 | TACAAAAGCTCTGGGGCCTA | CCAATTCCTCTGGGCAACT | 202 | 56 |
CAAS3 | (AG)7 | CTGGTGCGTAAGGTTGATGA | CAAACCACCACCAATCACAG | 132 | 53 |
CAAS4 | (CA)11 | ATTGCAAGTCCTGAGGCAAG | ATAATGGCGCCACAAAGTGT | 160 | 57 |
CAAS5 | (ACA)15 | TACATCAGTCCCGCAAATCA | CCATGTAGCCGATTCCACTT | 150 | 55 |
CAAS6 | (A)10 | TGCAAAGTAATTCCGAAACAA | CGCACATGAATTGGGGTAAT | 150 | 56 |
CAAS7 | (A)10 | GACCCAAGCCTTCACCACTA | TGTGTGGGATCCATTTTGAA | 200 | 59 |
CAAS8 | (AAC)14 | AATTTGTTCAGCATCTCGGG | CTGGTTGGTTCCTGGTGAGT | 150 | 56 |
CAAS9 | (AAC)9 | GTGATGCTTTGCCTGTGCTA | ATGGACGTTTGTAGGTGGGA | 200 | 56 |
CAAS10 | (AAG)5 | CTGTTCGTCATCATCATCGG | CGTAAATCAACCCCAACACC | 150 | 53 |
CAAS11 | (ACA)10 | TCCCGCTATTCTTGCTCTGT | GCTCAAAAATGCTTGTCTTTCA | 170 | 54 |
CAAS12 | (TGT)9 | GAGGAGGATCCCACAATGAA | GCCAAAAGAGCCATGGTAGA | 210 | 56 |
CAAS13 | (CAA)11aaatcccaaaaactgcaaattgtatgccatcttaaaccatac(CAA)7 | CAAAAATCCCAAAAACTGCAA | TCGATTTTTCGACTTGGGTC | 130 | 56 |
CAAS14 | (AAC)6 | CCGTAGATCTCAAAAACCATGA | GGAGGAAGGAAGCTCGAATC | 170 | 60 |
CAAS15 | (AAC)8 | AACCAACATCAATGGCATCA | TCTTTTCCTTTTTCCTCTTCCA | 140 | 60 |
CAAS16 | (CA)7 | TCAAATTTCCCTTTGCAAAAAT | GACCAAGGTCAACCACCTTT | 350 | 56 |
CAAS17 | (CA)8 | TCAAACACCTACACACCCACA | TCTCGGTCAATCTCACATGC | 250 | 56 |
CAAS18 | (CA)9 | ATGGGAGGGCAAATTTTAGG | AGTGAGTGGAGCGCTTGTTT | 350 | 56 |
CAAS19 | (CAA)6 | AACATTTTTCCAATCGAGGC | TGTAGGCTTACGGCCAAAGA | 200 | 56 |
CAAS20 | (CAT)5 | ACTGGAAAATCCCAATGCAC | AGCAAACTTGCACCCAACAT | 190 | 56 |
CAAS21 | (CTT)8 | GAATTTTCAAAACATGAGTCCCA | CCGGATCTGAAAAGACTTGC | 175 | 60 |
CAAS22 | (G)10 | TGATGAACAGAACTGCGCTC | ATTGGAGAGAGGCGAAATCA | 190 | 56 |
CAAS23 | (GA)6 | ACCGCATGCTAGGGAGTCTA | TGGGTGACTCACTTTTGTGG | 220 | 58 |
CAAS24 | (GA)6gca(AG)6(TG)8 | TCACTCACAAGCCACTAAGTCAA | GATGCGACACTATCCCCACT | 200 | 56 |
CAAS25 | (GT)15 | TCCATAATCAATTGGCTAAGCTC | AAGACTAACTCTCGACTGTATTTAGGC | 150 | 58 |
CAAS26 | (GT)7 | CGGCTTGGTTAACTGGATGT | TCTTCCTTTTCTTCAATGCG | 160 | 58 |
CAAS27 | (TA)6 | TTGGCATCATGCTCTAATCG | CTTGAAGTCGTGCCAGATGA | 280 | 60 |
CAAS28 | (TC)8 | CCATTGATGCAGGAAAGGAT | CAGCTTTGACAGCTCCAACA | 160 | 58 |
CAAS29 | (TCA)5 | TGCAAGTCAGTAGCCAAGACA | CTCGTCTCTCCTCATTCCCA | 180 | 58 |
CAAS30 | (TG)10 | GGTTTTTAGGTGATTTTCGCA | GCGAAACCTCGTATGGTTGT | 170 | 59 |
CAAS31 | (TG)12 | CAACGCGCTAGAGGAAGAAG | CCACTGCCCTAGCACACTAA | 160 | 56 |
CAAS32 | (TG)7 | TTTGGGGTACAACACTGGGT | CCTCACTCCTCTATATAAACAACACTT | 200 | 59 |
CAAS33 | (TGA)5 | GCAGTGATTCTGGCAGTGAA | TGCAGCAACATTTCCATCAT | 190 | 56 |
CAAS34 | (TGT)5 | TTTCTCGCAATTGTTCTCACA | TTCGATGAAATCCATCTTCTGA | 200 | 57 |
CAAS35 | (TTG)8 | AGGCAGAAGTTTGGAAGCAA | TCTCACTTCGGCTTCAGGAT | 180 | 56 |
CAAS36 | (A)11 | AGCACTAGAGTTCCAAGCCA | TTTTTATCGTTTCTTGTCACGC | 130 | 52 |
CAAS37 | (A)11 | CAACGCAAGAACACGTGAAT | TAGAGGCCAATTCAAGCCAT | 190 | 54 |
CAAS38 | (AAC)5 | CGCCTCAGAACCAAGTTCAT | TGCTTTGTTTTGGTTTTGTGA | 170 | 56 |
CAAS39 | (AAG)5 | CTGTTCGTCATCATCATCGG | CGTAAATCAACCCCAACACC | 170 | 54 |
CAAS40 | (AAG)6 | CCAAAGCCACTTCCAAACAT | TTCAGCCGGGCTTCTTTC | 110 | 54 |
CAAS41 | (AC)10 | GAAACCCACTTGGTCGTGTC | TTCATTTGGGTAGGCTCCAA | 190 | 56 |
CAAS42 | (AC)10 | CAAGTGTCGACGCAAGAGAT | TGACTTTTTGACTGCTCCCA | 250 | 56 |
CAAS43 | (AC)7 | GAGGAAGTGTGAAAGGTCGC | TCATTTTAAAGTGGTGTATGTGTGT | 170 | 54 |
CAAS44 | (AC)7 | ACACACACACGCACACACAC | CATGAACCTTTGATAGTTTTCCA | 150 | 56 |
CAAS45 | (AGA)5 | ATGGCTTTGACAAAAGGGAA | CTCCTTCACCCGACAATGTT | 180 | 57 |
CAAS46 | (AGA)6 | AGATCGCAGGCGTAGAAAGA | TGCTTCAACCACAACACCAT | 200 | 58 |
CAAS47 | (C)11 | CAAATTGGTTTGCATATCCG | AGCCCTTCACATCCATTGAG | 200 | 56 |
CAAS48 | (CA)10 | CCTCCTCCTTTAATTTGTGGC | TGAATCGTGAATGCTCTCTGA | 200 | 56 |
CAAS49 | (CA)10 | ACCTCCATAGCAGCAGCATT | GGCCAATTCTTAACGTGCTT | 140 | 56 |
CAAS50 | (CA)10 | CACTGGACCATTTTGCATTC | ATGAGATCCGGAGCAGATGT | 140 | 56 |
CAAS51 | (CA)11 | AAGCATTAAAACTCCCATAGCG | ATGTGTGCGTGTGTCATGTG | 140 | 52 |
CAAS52 | (CA)12 | CATTCCATGTTGCGTTTTTG | GGATAAGAGGGTGGTGGTGA | 200 | 56 |
CAAS53 | (CA)13 | GGCCCATTTGTTAAGGGTTT | AATGAGATCTGGCCTGGATG | 200 | 56 |
CAAS54 | (CA)6 | CCATTGGACCTCTTTGCATT | CCAGAGTGGATGATGATCTGA | 150 | 54 |
CAAS55 | (CA)6 | ACTCACATACACGCACACACA | AATGCTCTCATCCCTTTTGC | 150 | 56 |
CAAS56 | (CA)6 | CACATACACGCACACACACA | AATGCTCTCATCCCTTTTGC | 150 | 56 |
CAAS57 | (CA)8 | GCCCGAGACACTTTGGTTTA | CCAGAATGGATGAGGACCTG | 210 | 56 |
CAAS58 | (CA)9 | CTCCTGGTCCATGTATGAATGA | TGTGTGTATGTGTATGCGTGC | 150 | 54 |
CAAS59 | (CAA)10 | GGCCAACATAGGTGAGCATT | GTGTTGTAGGCCTTTGGTCC | 200 | 56 |
CAAS60 | (CAA)8 | ATGCAAAATGAAATGCGACA | TGTAGTTGTCTGTTTAATGGTTGTTG | 190 | 56 |
CAAS61 | (G)11 | AGAGGAAAAAGGCAAATGGC | CCCTTCATCAATCACACCAA | 130 | 54 |
CAAS62 | (GA)14 | AATGTTGGGACGGAGTTCAG | TTGTTGATTCATTCATCCCTTG | 130 | 56 |
CAAS63 | (GA)15 | CGCAGAGAAACACTCCATGA | GAAGTTGAATGTCATTTGTGTCAA | 100 | 56 |
CAAS64 | (GA)6 | AAAATATAATAAACAAAGCAAAAGTGC | CAGGTTTGTGGTTTCACCCT | 200 | 54 |
CAAS65 | (GA)6 | CGATATTCCTCGGTTTCCAA | CATGGGTCGTCTTCTCCACT | 200 | 54 |
CAAS66 | (GA)6 | CATCACTTTCCAGCCTGTCA | ATTTTCTGCCTCCCCTTTGT | 190 | 58 |
CAAS67 | (GA)7 | GGGTTTCAGAGAAAGGGGTC | CGCAAGCGTATTGGGTATTT | 130 | 56 |
CAAS68 | (GA)8 | ATGGAGGTTGCGATTTGAAG | CATCATCTCCACACTTTTTCCA | 130 | 54 |
CAAS69 | (GT)10 | ATTACAAATGTCGGTGCCGT | AGCACAACGATAAGATGATATGC | 170 | 54 |
CAAS70 | (GT)8 | TCGCGATAGAGGTTTTGGAA | AACAACAACGATTCATCACAAGA | 200 | 56 |
CAAS71 | (GTT)15 | CCATGTAGCCGATTCCACTT | TTCGGCAACGTAGGAAAAAT | 160 | 54 |
CAAS72 | (T)10 | TTTTCCAGTGTCAACCCATCT | ACATGAGGCCAAAAACTGCT | 170 | 54 |
CAAS73 | (TG)13 | TTGCACCTCTGTTGAAGACG | TCACCAACACTCTAATCCTCAATC | 190 | 54 |
CAAS74 | (AC)6 | CCCACCGTATTACACAAGGG | GCGAGGAAGAAGATGACGTT | 200 | 56 |
CAAS75 | (AG)15 | TCGATTGCACAATAAATGGTTT | GAGGTCGACTCCCATTGAAA | 180 | 54 |
CAAS76 | (AG)6 | GCCTGTTAATGAGAAGAACTGGA | TTTCAAAATTTAGTTTCTCTCTGTCTC | 200 | 56 |
CAAS77 | (CA)21 | TAGCAGCCAACAATCAGTGG | GGTGATGTTGCTCATGTTCG | 180 | 56 |
CAAS78 | (CA)7 | TCAAATTTCCCTTTGCAAAAAT | TCGAACACAACTTCTTCATTTCTC | 180 | 56 |
CAAS79 | (CA)7 | TCAAATTTCCCTTTGCAAAAAT | CATGGAAAATCTTTTATTTTGTGTG | 100 | 58 |
CAAS80 | (CA)8 | GTGTGAAAACTCACCCGGTC | TGTGTGTAAGTGTGTGTATGTGTGTG | 130 | 54 |
CAAS81 | (GA)15 | AACTTACAGGGGCCACACAC | TGTGCATTATACTTTACGTATGTTCCT | 100 | 52 |
CAAS82 | (GA)17 | TTTGCTTGACAATGGTGGAA | ATTCAACAAGCAAGGGTTGG | 120 | 52 |
CAAS83 | (T)10 | GATTTGCGTTTAGGGTTCCA | GAACAAACTACGTTTTATTGTCCAGA | 180 | 52 |
CAAS84 | (TA)6 | TGTCGACACCACAGCTATTTT | TGTGGTTCGTTGTTTTGGTG | 200 | 56 |
CAAS85 | (TCA)6 | TTGAAGTGAATAAGATGAAGAAGTGT | GTTGCCTTTCCTTGCATGAT | 130 | 56 |
CAAS86 | (TG)10 | TCGCGATAGAGGTTTTGGAA | CACAAACAACAACGATTCATCA | 200 | 56 |
CAAS87 | (TG)14 | CTCTACCATGGGCCATTTCT | AGAGATAGAGAGAGAGACAGAGATGAA | 90 | 54 |
CAAS88 | (TG)18 | TCCTACCGATCTCTCTCTCCC | GTGGCATAACCGCGTAAGTT | 130 | 56 |
CAAS89 | (TG)18 | TGTCTCGCCTTCAATCTTCC | CTTGCTAAGTGAGACTGCTGCT | 190 | 54 |
CAAS90 | (TG)19 | TCCATAGTCGATGAGGACCG | TTGTCTCATTGTCTTTCTTTTCTTTC | 100 | 54 |
CAAS91 | (TG)6 | ATCTTCGGCTTGGTTGATTG | GAGGCGGCCACATTAGACT | 200 | 56 |
CAAS92 | (TG)9 | CGAGATCTGGAGTGGATTTAGA | TTTTCATATGCCACATGCTCA | 170 | 56 |
CAAS93 | (TTC)5 | GGCATTGCTTACTTACCGGA | CGACGTCGACATTAACATGC | 200 | 56 |
CAAS94 | (TTG)9 | TCCTCAACACGTGATGCAAT | TGTAGGACCAGGAAGGTCGT | 180 | 56 |