Figure 5

Schematic representation of the binary vectors p6-HSP-TP-OCS (A) and p7N-ATDs- rolC (B) used for the transformation experiments. (A) Structure of the plasmid containing the Ac transposase and heat shock promoter (HSP) Gmhsp17.5-E from soybean [42], and hpt as plant selectable marker. (B) Structure of the activation construct (ATDs) [26]. The ATDs contains hpt as transposition selection marker. The rolC gene is outside of ATDs and the CaMV 35S promoter on the left site of the ATDs keeps this gene active under non-excised stage. The nptII gene with the nopaline synthase promoter (nos) is used as transformation marker. Relevant restriction enzyme cutting sites are introduced into the maps. (P-NOS = Nopaline synthase promoter; HSP = GmHSP 17.5-E promoter; nptII = neomycin phosphotransferase gene; hpt = hygromycin phosphotransferase gene; 35S = CaMV35S promoter; T35S = CaMV35S terminator; OCS = Octopine synthase gene terminator; EN4 = four tandem repeats of enhancer fragments; rolC = rolC gene from Agrobacterium rhizogenes; LB and RB = Left- and right border sequences).