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Table 1 Copy number of HSP::TRANSPOSASE in the single transgenic lines, and genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.

From: Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery

Transgenic line

Copy number

Genomic insertion locus (scaffold:position)*

Score

E- value

Transcript

N66-2

1

3:16,990,223

1132.0

0

POPTR_0003s17690 (no functional annotation)

N66-5

1

3:15,414,346

1222.2

0

POPTR_0003s15650 CTP synthase (UTP-ammonia lyase)

  1. *based on BLAST-results against the genome sequence of P. trichocarpa v2.0 (Phytozome v7.0; http://www.phytozome.net/poplar). Successful positioning of blasted sequence on the physical map of P. trichocarpa was assigned to the Populus-aspen genome because of the high collinearity between the P. trichocarpa and P. tremula/P. tremuloides genomes [49].