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Table 3 Heat shock treatment experiment, treatment conditions, transgenic lines treated and number of in vitro rooted plants cultivated in greenhouse

From: Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery

Heat shock experiment

Treatment conditions

Transgenic lines treated

Rooted plants phenotyped in greenhouse

1

42°C for 16 to 24 hours

N82-2, N82-3, N82-4, N82-5, N82-7, N82-10, N82-11, N82-12, N82-14, N82-15

7,856

2

42°C for 24 hours

Esch 5 (control), N 92-1, N 92-2, N 92-3, N 92-4, N 95-1, N 95-2, N 95-3, N 95-4, N 95-5, N 95-6

623

3

Three days at 42°C for 8 hours, recovering over night

N82-2, N82-5, N82-7, N82-11, N82-14, N82-15

1,587

4

42°C for 24 hours

N 92-1, N 92-2, N 92-3, N 92-4, N 92-5, N 92-6, N95-1, N 95-2, N 95-3, N 95-4, N 95-5, N 95-6

2,017

   

12,083