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Figure 1 | BMC Genomics

Figure 1

From: Pre-capture multiplexing improves efficiency and cost-effectiveness of targeted genomic enrichment

Figure 1

Experimental design showing pertinent steps in the TGE protocol including the indexing PCR during which barcodes are incorporated into separate DNA libraries. A) standard post-capture TGE, 96 captures are required as barcodes are incorporated during second PCR after capture and prior to sequencing, B) pre-capture multiplexing with pools of 12 (pre-capture 12a and pre-capture 12b), 8 captures required as barcodes are incorporated when adaptors are ligated prior to the first PCR and capture, and C) pre-capture multiplexing with pools of 16 (pre-capture 16), 6 captures required.

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