Figure 2

RNA binding proteins identified by incubation of labeled RNAs to human protein microarrays. (A) Pfam protein family domains and motifs present in proteins bound to at least 1 RNA (B) Gene ontology (GO) terms present in the same population of RNA binding proteins from (A). (C) Binary heat map representation of the RNA binding capacity to protein microarrays. Human coding and non-coding RNAs used in incubation to the microarrays are displayed in columns. The 137 out of 9400 total proteins bound by sense but not antisense strand RNA samples with ≥ 2.5-fold above background intensity and Z-Score ≥ 3 are displayed in rows. Stau1 and CNBP protein localization indicated with arrows. (D) Image and quantification of human protein microarray showing selective binding signal of TP53 mRNA sense strand to duplicate Stau1 protein spots. The binding signal is shown with respect to adjacent proteins spotted in the same sub-array.