Figure 2From: Novel β-catenin target genes identified in thalamic neurons encode modulators of neuronal excitabilityGene profiling in the forebrain. Scatterplots show mean gene expression fold changes between the thalamus, hippocampus, and cortex by RT-qPCR. p values (Student’s t-test) revealed statistical significance for all fold changes > 2 and < 0.5. A logarithmic scale is used. Red frames surround the plot areas of at least two-fold higher expression in a given brain region compared with the other two regions, gray frames - of at least two-fold lower. (A) Expression of VGCC genes in the thalamus vs. cortex (x-axis) and hippocampus (y-axis). (Left plot) VGCC genes defined as putative LEF1/TCF targets. (Right plot) Remainder of the VGCC genes. The proportions of highly expressed genes in the group of putative LEF1/TCF targets (left plot) and in the non-target group (right plot) were compared using Fisher’s Exact test, indicating a nonrandom association (p = 0.021). (B) Expression of all putative neuronal LEF1/TCF targets in the thalamus vs. cortex (x-axis) and hippocampus (y-axis). Notice that many genes are highly expressed in the thalamus. (C) Expression of all putative neuronal LEF1/TCF targets. (Left plot) Cortex vs. thalamus (x-axis) and hippocampus (y-axis). (Right plot) Hippocampus vs. thalamus (x-axis) and cortex (y-axis). Notice that this group of genes is not preferentially expressed in the cortex or hippocampus. n = 6 independent biological samples.Back to article page