Figure 4

Positions of LEF1/TCF motifs in the conserved noncoding sequences in the genomic flanks of the transcription start sites of genes selected for experimental validation. The schemes represent Drd3, Gabra3, Glra1, Grid2, Cacna1g, Cacna2d2, Kcna6, Kcnh8, and Calb2 genes. The plots are on the gene strand, nucleotide positions are given relative to the transcription start site (TSS) of each gene, as defined in the Ensemble version used. For Gabra3, an alternative TSS prediction, marked with the asterix, based on the NCBI Reference Sequence NM_017069.3, is also shown. CNSs are represented as green rectangles. Positions of LEF1/TCF motifs (analyzed only in the CNSs) are marked as red bars, above or below the axis depending on the strand. Exons within the analyzed flanks (for majority of the genes – only the first exons) are shown as black rectangles on the axis. Amplicons used in the ChiP assay are shown as numbered thick black lines.