Figure 5

ChIP analysis of histone acetylation and β-catenin binding to LEF1/TCF motifs of the candidate target genes. (A) The graph shows the mean percentage of input chromatin precipitated with an anti-H3Ac antibody. Fragments of the Gapdh promoter (Gapdh-P) and exon (Gapdh-E) were used to determine the signal levels in the case of open and closed chromatin, respectively. The blue area indicates the level of signal for closed chromatin, assessed based on the precipitation of the exonic fragment of Gapdh. (B) Mean percentage of input chromatin precipitated with an anti-β-catenin antibody. The blue area indicates the level of background, determined with normal IgG. In some cases β-catenin binding to chromatin was not detected (ND). n = 4 independent biological samples. Error bars indicate SD. ***p < 0.001, **p < 0.01, *p < 0.05 (ANOVA).