Figure 3

Neighbor-joining phylogeny of Hsfs from M. domestica, P. trichocarpa and A. thaliana. The phylogenetic tree was obtained using the MEGA 5.0 software on the basis of amino-acid sequences of the N-terminal domains of MdHsfs including the DNA-binding domain, the HR-A/B region and the linker between both regions. The final dataset included a total of 281 positions. Evolutionary distances were computed using the Jones-Taylor-Thornton matrix-based method and by removing all ambiguous positions for each sequence pair. Numbers indicate bootstrap values >80 based on 1000 replicates for the major nodes. The abbreviations of species names are as follows: Md, Malus domestica; Pt, Populus trichocarpa; At, Arabidopsis thaliana.