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Figure 4 | BMC Genomics

Figure 4

From: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust

Figure 4

Western blot analysis with α- Rubisco antibody. (A) Western blot of five micrograms of proteins extracted from the two biological replicates of Bowman (Bow) and Bowman-Rph15 (Bow-Rph15) at 4 days after mock inoculation (C) or after inoculation with leaf rust spores (I) were separated on 12% SDS-PAGE and hybridized with a Rubisco antibody. Bands representing the main Rubisco degradation forms are numbered from 1 to 5 on the Western right side. (B) Quantification data of Rubisco degradation forms; evaluation of degradation forms was conducted for the five bands indicated in (A) in Bowman (Bow) and Bowman-Rph15 (Bow-Rph15) under control (C) or after inoculation with leaf rust spores (I) conditions. The data are the mean of three independent experiments. Different letters on the bars indicate significant difference P-values (P < 0.05) of the t-TESTs performed comparing control and inoculated samples.

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