Figure 1

Eight distinctly different PPARβ/δ-dependent mechanisms of transcriptional regulation. (A) 612 genes were categorized into eight different response types. Relative expression was based by comparison with control, wild-type mouse keratinocytes. White indicates basal expression; red indicates higher expression compared to control, wild-type mouse keratinocytes; green indicates lower expression compared to control, wild-type mouse keratinocytes. An up arrow indicates repression and a down arrow indicates activation. The number of genes within each category is shown in the bar graph. (B) Enrichment of functional gene categories of the 612 differentially regulated genes within each of the response types. Statistically significant enriched gene categories were identified by PANTHER (P ≤ 0.05) and are indicated by a box, with darker shaded boxes depicting lower P values. (C) Comparison of gene expression profiles observed with disruption of PPARβ/δ (y axis) and activation with GW0742 in wild-type keratinocytes (x axis) for the 612 genes as categorized by response type. Relative gene expression levels were log transformed and a ratio relative to control wild-type keratinocytes was plotted for each gene. (D) Violin plots of the log transformed ratios of gene expression in wild-type keratinocytes treated with GW0742 compared to Pparβ/δ^−/− keratinocytes for the type V and VI responses. Each data point represents the mean of at least three independent biological replicates. The mean ratio is shown next to each violin plot.