Table 5 Description of 95 unique S . sclerotiorum EST contigs with putative involvement in virulence or pathogenicity

4 genes involved in the recognition of the host and in signaling pathway

1457

c2h2 type zinc finger containing protein

222

2.2E-35

75.5%

1796

guanine nucleotide-binding protein alpha subunit

171

2.7E-17

95.0%

3623

importin beta-2

109

1.2E-09

85.7%

6785

c2h2 transcription factor

135

2.8E-19

79.9%

18 genes affecting biosynthesis and the integrin of fungal cell walls

330

alpha-1,2-mannosyltransferase kre5

196

8.4E-19

70.9%

2296

cell wall biogenesis protein glutathione transferase

122

2.8E-14

90.5%

2424

endoglucanase-4 precursor

326

6.4E-27

76.0%

2930

alpha-1,2-mannosyltransferase alg11

154

1.7E-22

95.3%

3362

alpha-glucan-branching enzyme

234

6.8E-29

82.6%

3825

alpha-mannosyltransferase

248

3.4E-41

71.1%

4843

dolichol-phosphate mannosyltransferase

253

1.9E-34

87.1%

5737

endoglucanase ii

122

8.7E-16

86.0%

5773

dolichol-phosphate mannosyltransferase

255

1.9E-26

89.7%

6654

endochitinase 42

189

1.5E-28

87.0%

6797

chitinase

105

2.3E-11

79.9%

6956

endoglucanase ii

267

6.8E-45

86.7%

7397

gpi-anchored cell wall beta-1,3-endoglucanase

159

3.0E-24

69.3%

8474

af215732_1class iv chitin synthase

113

2.0E-07

86.0%

8681

chitin synthase

138

2.0E-20

95.6%

8785

mannan polymerase ii complex anp1 subunit [61]

173

8.6E-24

92.8%

9176

gpi-anchored cell wall beta-1,3-endoglucanase

295

9.2E-34

66.5%

9570

mannosyl transferase

122

3.3E-15

82.6%

12 genes involved in the production of infection structures

97

siderophore biosynthesis

106

5.9E-12

81.5%

239

mannosyl transferase

221

1.3E-22

94.3%

253

adenylate kinase

140

6.3E-19

88.3%

427

serine threonine-protein phosphatase pp2a catalytic subunit

289

4.9E-51

99.4%

1554

adenylate cyclase

135

1.4E-18

83.2%

4181

mfs toxin efflux pump

194

1.1E-21

69.4%

5419

alcohol dehydrogenase

190

3.4E-28

75.7%

5981

adp-ribosylation factor 1

455

7.3E-23

93.7%

6192

adp-ribosylation factor 1

103

3.5E-12

100.0%

6325

nadp-dependent alcohol dehydrogenase

347

4.0E-53

82.8%

7407

1-phosphatidylinositol phosphodiesterase

102

9.2E-05

76.0%

9579

alcohol oxidase

112

9.7E-15

90.7%

39 genes involved in the penetration of the cuticle and cell wall

47

cellulose 1,4-beta-cellobiosidase

165

7.3E-23

77.1%

473

xyloglucan-specific endo-beta-1,4-glucanase precursor

228

7.6E-36

84.0%

485

cutinase

375

2.5E-63

68.5%

555

endo-1,4-beta-xylanase

265

3.8E-19

79.3%

717

endo-1,4-beta-xylanase

348

2.8E-62

78.4%

970

acetyl xylan

118

2.3E-16

73.5%

1533

endopolygalacturonase 2

181

1.3E-27

89.5%

1562

carbohydrate esterase family 1 and carbohydrate-binding module family 1 protein

241

4.4E-28

76.1%

2427

4-coumarate:CoA ligase

185

1.8E-29

79.8%

2450

acetolactate synthase

236

1.9E-39

93.3%

3006

aspartic-type endopeptidase

171

1.5E-23

71.2%

3115

fungal alpha-l-arabinofuranosidase

110

3.1E-13

91.6%

3147

extracellular exo-polygalacturonase

269

1.5E-12

83.5%

3513

beta-xylosidase

219

9.1E-37

84.6%

3712

feruloyl esterase b

187

1.7E-27

78.6%

3861

pectin methylesterase

170

2.4E-26

80.0%

3907

extracellular endo-1,5-alpha-l-arabinosidase A

172

1.6E-25

80.0%

4037

aspartic endopeptidase

129

3.2E-18

82.4%

4295

cutinase

278

2.6E-36

66.3%

4446

xyloglucan-specific endo-beta- -glucanase precurso

626

8.3E-89

77.3%

5554

beta-glucosidase 1b

267

8.0E-46

83.7%

5562

alpha-l-arabinofuranosidase

130

1.1E-15

74.2%

5679

polygalacturonase 1

427

9.8E-60

96.6%

5918

acetyl xylan esterase

345

5.1E-16

84.0%

6412

exoglucanase 2 precursor

141

4.8E-19

90.3%

6383

alpha-L-arabinofuranosidase A

130

1.1E-13

78.3%

6431

exopolygalacturonase

135

1.3E-16

89.0%

6759

endo-1,4-beta-xylanase

359

8.8E-45

81.1%

6824

pectin methylesterase

107

3.1E-13

79.7%

7085

pectinesterase family protein

109

7.7E-12

82.5%

7661

polygalacturonase 1

601

3.9E-45

93.2%

7834

extracellular exo-polygalacturonase

386

3.0E-61

68.0%

8501

pectin methylesterase

252

1.6E-41

62.5%

9093

carbohydrate esterase family 8 protein

244

9.4E-31

71.6%

9509

extracellular endo-1,5-alpha-l-arabinase

322

2.0E-36

74.7%

9525

cellulase family protein

239

1.8E-37

72.8%

9583

acetolactate synthase

123

1.3E-16

90.6%

9655

acetyl xylan esterase

129

2.3E-16

82.8%

10069

endopolygalacturonase 2

460

1.2E-73

91.7%

9 genes involved in responding to host immune system

1220

efflux transporter

120

1.9E-15

80.3%

4783

abc bile acid transporter

142

1.6E-17

72.5%

5659

mfs transporter

236

8.0E-38

80.8%

6180

ATP-binding cassette transporter

229

1.7E-40

81.1%

6538

glutathione transferase

486

3.7E-91

70.2%

7926

glutathione s-transferase ure2-like protein

123

1.3E-16

79.8%

8029

ornithine decarboxylase

234

9.5E-39

90.3%

8188

glutathione s-transferase

527

6.5E-100

79.7%

9716

salicylate hydroxylase

202

5.4E-34

85.8%

3 genes involved in fungal nutrition (virulence-associated)

1446

methionine aminopeptidase 2

337

7.2E-47

84.8%

1889

vacuolar atpase proteolipid subunit c

314

2.6E-23

92.5%

5131

vacuolar atpase proteolipid subunit c

157

3.1E-21

90.4%

Others 10 genes related with pathogen virulence or pathogenicity

145

acid proteinase

583

1.8E-89

63.5%

1387

methylcitrate synthase precursor

182

1.7E-27

96.2%

2840

centromere kinetochore protein

217

4.1E-34

73.0%

3499

vacuolar protein 8

255

7.7E-28

97.4%

3747

acid proteinase

246

6.5E-40

66.9%

5648

dipeptidyl peptidase

226

1.2E-12

83.1%

7192

alpha-amylase precursor

207

2.9E-32

62.7%

7273

vanillyl-alcohol oxidase

146

5.3E-21

80.3%

10090

phospholipase d active site motif protein

235

3.3E-39

69.9%

10153

alpha-ketoglutarate dependent xanthine dioxygenase

249

1.2E-41

87.6%