Skip to main content

Table 1 Semi-quantitative analysis of SCGB1A1 RT-PCR amplicons

From: Multiple secretoglobin 1A1 genes are differentially expressed in horses

  

Relative amplicon density

Expression category

Tissue

GAPDH

SCGB 1A1

SCGB 1A1A

n

High

Lung

1.00

3.50 ± 0.94

3.65 ± 0.72

5

 

Uterus

1.00

2.25 ± 0.59

2.02 ± 0.61

4

 

Fallopian tube

1.00

2.67 ± 1.60

1.49 ± 1.59

3

 

Mammary gland

1.00

0.67 ± 0.67

nd - 0.33

3

 

Ovary

1.00

nd - 0.65

nd - 0.07

3

 

Prostate

1.00

nd - 1.67

nd

2

Low

Aorta

1.00

0.42 ± 0.55

nd - 0.32

4

 

Urethra

1.00

0.25 ± 0.38

0.01 ± 0.01

4

 

Epididymis

1.00

nd - 0.07

0.16 ± 0.20

2

 

Skin

1.00

nd - 0.49

0.04 ± 0.01

4

 

Trachea

1.00

nd - 0.19

0.81 ± 0.73

4

 

Eye

1.00

nd - 0.26

nd - 0.43

4

 

Brain

1.00

0.09 ± 0.08

0.08 ± 0.02

4

 

Nose epithelium

1.00

nd - 0.17

0.04 ± 0.03

4

 

Bladder

1.00

0.06 ± 0.04

nd - 0.03

4

 

Spleen

1.00

nd - 0.07

nd - 0.04

4

 

Kidney

1.00

nd - 0.02

nd - 0.01

4

 

Liver

1.00

nd - 0.02

nd - 0.01

4

 

Tongue

1.00

nd - 0.05

nd

3

 

Small intestine

1.00

0.02 ± 0.01

nd

4

 

Large intestine

1.00

0.03 ± 0.02

nd

4

 

Pituitary

1.00

nd - 0.01

nd - 0.02

4

 

Salivary gland

1.00

nd - 0.01

nd

4

 

Adrenal gland

1.00

nd - 0.01

nd

4

 

Seminal vesicle

1.00

nd - 0.42

nd

2

 

Testis

1.00

nd

0.02 ± 0.01

2

Absent

Eyelid gland

1.00

nd

nd

4

 

Cardiac muscle

1.00

nd

nd

4

 

Thyroid

1.00

nd

nd

4

 

Stomach

1.00

nd

nd

4

 

Pancreas

1.00

nd

nd

4

 

Lymph node

1.00

nd

nd

4

 

Bone marrow

1.00

nd

nd

3

  1. Quantification of gene-specific SCGB1A1 cDNA amplicon intensity relative to GAPDH using Image Lab™ software 2.0.1; nd, non-detected.