Probe sequence | Type of synthesis error(s) |
---|---|
5’ C12T11A10C9C8G7T6A5C4C3G2T1 3’ | Full length probe (no error) |
5’ C8G7T6A5C4C3G2T1 3’ | Incorporation error base 9 |
5’ C8G7T6_5C4C3G2T1 3’ | Incorporation error base 9 and abasic site |
5’ C12T11A10_9C8G7T6_5C4C3G2T1 3’ | Full length probe with two abasic sites |
5’ T6_5C4_3G2T1 3’ | Incorporation error base 7 and two abasic sites |