Figure 2

Insert size and depth distribution of YH Tsp 45I RRL. (A) Depth distribution of target region in Tsp 45I RRL. The red dashed line shows standard Poisson distribution. (B) Insert size distribution in Tsp 45I RRL. Insert size was calculated based on aligned paired end reads in Tsp 45I RRL sequencing data. Compared to simulation results, these fragments shorter than 400 bp were over-represented and longer fragments were under-represented. These peaks along the distribution indicated the accumulation of repeat sequence.