Figure 3
Disruption of MLO2 compromises P. syringae virulence. A. The virulent pathogen Pto DC3000 was pressure-infiltrated into the leaves of Arabidopsis Col-0 or T-DNA insertion lines in putative interactors of HopZ2C/A. An insertion in At1g11310 (mlo2-7) results in increased resistance to Pto DC3000. * indicates significant difference from Col-0 by Fisher's Protected Least Significant Difference (PLSD) test. Error bars indicate one standard deviation of the mean. B. Schematic showing the MLO2 gene and protein structure with the point of insertion for the T-DNA insertion lines and the point mutant. The left side shows the gene with introns represented as lines and exons represented as boxes. MLO2 has two splice forms which differ at the 3' end of the gene. The right side shows the transmembrane structure of the MLO2 protein. mlo2-6 and mlo2-7 are T-DNA insertion lines while mlo2-11 is a point mutant. MLOΔ1-280 indicates the clone identified in the cDNA library screening. C. The weak pathogen Pci 0788-9 carrying the empty vector (Ev) or HopZ2 was pressure-infiltrated into the leaves of Arabidopsis Col-0 or mlo2-7. Statistical significance was determined as stated in part A.