Figure 4
HopZ2C/A, but not HopZ1c, interacts with MLO2Δ1-280 in planta by bimolecular fluorescence microscopy. Agrobacterium carrying HopZ2C/A::nYFP or MLO2Δ1-280::cYFP (or the reciprocal combination) were mixed at equivalent optical densities and pressure-infiltrated into the leaves of N. benthamiana. Expression of the proteins was induced by 20 μM dexamethasone. Sections of leaf tissue were imaged with a Leica SP5 confocal scanning microscope 72-96 hours post-induction. The close-up images in the second column show the reticulate pattern reminiscent of the endoplasmic reticulum. The scale bar indicates 100 μm.