Computational discovery and RT-PCR validation of novel Burkholderia conserved and Burkholderia pseudomallei unique sRNAs

Table 3 List of RT-PCR validated sRNA genes in conserved in Burkholderia and unique to Burkholderia pseudomallei.

	Name	Chr/Strand	Start - end/Length	GC content	Conservation (Infernal search)
*Highly conserved in Burkholderia*
	Bp1_Cand449_SIPHT*	1/-	110185 - 110354/170	50.59%	Bacteria (detected in Proteobacteria, Bacteroidetes, Firmicutes, etc)
	Bp1_Cand612_SIPHT	1/-	2290411 - 2290508/98	52.04%	Burkholderia
	Bp1_Cand684_SIPHT	1/-	2768674 - 2768787/114	64.04%	Bacteria (detected in Actinobacteria, Cyanobacteria, Firmicutes, etc)
	Bp1_Cand697_SIPHT	1/-	2887980 - 2888055/76	64.47%	Burkholderia
	Bp1_Cand738_SIPHT	1/-	3154052 - 3154260/209	50.72%	Burkholderia
	Bp1_Cand871_SIPHT^	1/+	4031759 - 4031986/228	59.21%	Burkholderia
	Bp2_Cand287_SIPHT	2/-	2326038 - 2326224/187	62.57%	Proteobacteria (predominantly in Burkholderiales, detected in Deltaproteobacteria and Gammaproteobacteria)
*Unique to B. pseudomallei*
	Bp2_Cand11_sRNAscanner	2/-	892370 - 892562/193	36.27%	B. pseudomallei
	Bp2_Cand77_SIPHT	2/+	575285 - 575425/141	57.45%	B. pseudomallei

* Bp1_Cand449_SIPHT and Bp1_Cand846_SIPHT were verified using the same pair of primers with only Bp1_Cand449_SIPHT also confirmed via sequencing.
^ Bp1_Cand871_SIPHT was not confirmed via sequencing due to ambiguous sequencing results.

ISSN: 1471-2164