Figure 4

Phylogeny of DM-domain containing genes based on amino-acid sequence conservation. The evolutionary history of DM-domain containing genes was inferred by using the Neighbor-Joining method. The percentage of replicate trees in which the associated genes clustered together in the bootstrap test (1,000 replicates) is shown next to the branches (Bootstrap values below 70 percent are not shown). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The analysis involved 55 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 62 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 [45]. Red spot indicates duplication period of dsx gene duplication in cladocerans.