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Table 1 Library mutants identified as EcoRI-sensitive in MATα cells , MATα cells , or both MATα and MATα strains a,b

From: A multistep genomic screen identifies new genes required for repair of DNA double-strand breaks in Saccharomyces cerevisiae

Mutant

MATα

MATa

Mutant

MATα

MATa

mre11 c

S

S

rad54

S

S

rad50

S

S

rad55

S

S

rad51

S

S

rad57

S

R

rad52

S

S

xrs2

N/D

S

adk1

S

S

mrps35

S

N/D

ado1

S

S

not5

SS

SS

akr1

SS

S

nup84

S

SS

apq13/net1

SS

S

och1

S

N/D

arp5

N/D

S

psy1/ykl075c

S

R

atp2

SS

S

rad5

S

R

bck1

SS

S

rtt109(rem50) d

S

S

bik1

N/D

S

rpb9

S

SS

bud19/rpl39

SS

SS

rpl31a

S

S

bud30/rpc53

N/D

SS

rsm7/yjr114w

N/D

S

bud32

N/D

S

rtf1

N/D

SS

bur2

S

SS

rvs161

S

SS

cax4

SS

SS

sae2

S

S

ccr4

N/D

S

sam37

SS

SS

cdc40

N/D

SS

sco1

N/D

SS

cgi121

SS

SS

sfp1

SS

S

cis3

SS

S

slm4

S

S

cnm67

SS

S

spt10

S

S

ctf4

SS

SS

spt20

S

R

ctf8

SS

S

srv2

S

S

dcc1

SS

SS

taf14

SS

S

ddc1

S

S

trm9

S

SS

eaf1/opi7

S

N/D

tsr2/ylr434c

S

S

exo1

S

S

ubp8

S

SS

gcn5

SS

S

ubr1

S

N/D

gnd1/yhr182c-a

SS

SS

ume6

SS

S

hsp150

SS

S

vma7

SS

S

htl1

SS

SS

vph2/ykl118w

SS

S

ids2

S

SS

ybr099c/mms4

SS

S

img2

SS

N/D

ydr417c/rpl12b

S

N/D

lip5

S

S

ydr433w/npl3

SS

N/D

lrp1

SS

S

ygl218w/mdm34

N/D

SS

lsm7

S

SS

ylr235c/top3

S

S

mct1

S

SS

yml009w-b/spt5 e

SS

S

mms2

S

S

yml012c-a/ubx2

S

S

mms22

SS

SS

ynr068c

S

S

mms4/ybr099c

SS

SS

   
  1. a Eight RAD52 group mutants are shown at the top of the table. Mutants were ranked as resistant (R), sensitive (S), or strongly sensitive (SS) (see Methods). N/D usually indicates that the library strain was unable to grow on galactose plates. Some genes could not be tested for other reasons, e.g., MATα cdc40 cells were Ura+.
  2. b Gene names separated by a forward slash indicate deletions affecting two or more overlapping open reading frames. The coding region of the gene listed first was deleted in each strain.
  3. c The MATα mre11 strain was reconstructed for this study because the library mutant did not exhibit phenotypes characteristic of mre11 mutants (MMS- and gamma-sensitivity). dRTT109 is usually referred to as REM50 in the literature. eyml009w-b overlaps the verified gene spt5 and another open reading frame called yml009c-a.