Massively-parallel sequencing of genes on a single chromosome: a comparison of solution hybrid selection and flow sorting

Table 2 Sequence metrics

	Total reads	Total bases	Filtered reads	Filtered reads on X	Realigned reads on X	Realigned bases on X	Total mean base coverage
SHS	20,896,079	752,258,844	15,998,676	7,493,424	7,724,895	278,096,220	247.0x
SHS-PE	20,893,298	1,587,890,648	18,806,032	8,230,111	8,242,645	626,441,020	521.4x
SHS-PE Low	1,814,872	137,937,872	1,633,726	714,915	716,084	54,422,384	45.3x
FS	92,197,660	7,007,022,160	74,367,412	24,835,187	26,040,307	1,979,063,332	45.2x

Read counts for sequence data from Solution Hybrid Selection (SHS), SHS paired end (SHS-PE), SHS paired end low (SHS-PE low) and Flow Sort (FS) capture experiments. Absolute number of read counts and base counts are shown. Total mean base coverage is (total sequenced bases/total targeted bases).

ISSN: 1471-2164