Figure 5
Validation of differentially expressed genes with Real-time RT-PCR. A. The relative mRNA abundance of retinal photosensitivity genes (Rgr, Rdh8, Crbp and Gc) in feeders and nonfeeders was determined by Real-time RT-PCR. Compared with nonfeeders, the mRNA levels of Rgr and Crbp were significantly increased, whereas Gc was decreased significantly. B. The relative mRNA abundance of clock genes (Per1, Per2, Clock and Bmal1) in feeders and nonfeeders was determined by Real-time RT-PCR. Compared with nonfeeders, the mRNA levels of Per1 and Per2 were decreased significantly and slightly, respectively, whereas Clock and Bmal1 were slightly increased. C. The relative mRNA abundance of appetite control genes (Pomc, Pyy, leptin, Npy and ghrelin) in feeders and nonfeeders was determined by Real-time RT-PCR. Compared with nonfeeders, the mRNA levels of Pomc, Pyy and leptin were significantly increased, whereas Npy and ghrelin were decreased significantly and slightly, respectively. D. The relative mRNA abundance of learning and memory genes (Creb, Ncam, c-fos and Bdnf) in feeders and nonfeeders was determined by Real-time RT-PCR. Compared with nonfeeders, the mRNA levels of Creb and Ncam were slightly decreased, c-fos and Bdnf were decreased significantly. Data are presented as mean ± standard error (n = 6). * indicates significant differences between groups based on one-way analysis of variance (ANOVA) followed by the post hoc test (P < 0.05).