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Figure 4 | BMC Genomics

Figure 4

From: Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the σ54 (RpoN) regulon of Salmonella Typhimurium LT2

Figure 4

Promoter location, orientation, and activity for selected σ54binding sites. The ratio of β-galactosidase activity (Miller Units) in WT+DctD250 vs. ΔrpoN+DctD250 cells is shown for (A) known (light blue bars) and predicted (dark blue bars) σ54-dependent promoters, and (B) potential intragenic promoter sequences (red bars) in the promoter reporter vectors, pDS11 or pDS12 (black bars). Double asterisks denote significant increase in β-galactosidase activity in WT+DctD250 versus ΔrpoN+DctD250 (p-value <0.02). Circled numbers below locus tags indicate orientation of the potential promoter sequence, as illustrated in (C). Orientation of potential intragenic promoter sequence is: 1) same as ORF and >300 bp from 3’ end; 2) same as ORF and <300 bp from 3’ end of a convergent downstream gene; 3) opposite of ORF and >300 bp from 5’ end; 4) opposite of ORF and <300 bp from the 3’ end of an upstream gene; and 5) opposite of ORF and <300 bp from 5’ end of gene, but >300 bp from the 3’ end of an upstream gene.

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