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Table 1 DctD250-dependent activity of predicted and potential σ 54 -dependent promoters

From: Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the σ54 (RpoN) regulon of Salmonella Typhimurium LT2

  

Miller unitsb

 

Promotera

WT

WT+DctD250

WT-N

Intergenic:

   

STM0462 (glnKamtB)

1.1 ±0.1

180 ±22

1778 ±28

STM3521 (rtcBA)

14 ±6.8

220 ±55

N.D.

Intragenic:

   

STM0699

5.3 ±1.6

56 ±6.2

N.D.

STM2430

19 ±3.8

90 ±5.5

N.D.

STM2939

2.2 ±1.4

76 ±17

N.D.

Controls:

   

IS492-CJ (pDV6)

6400 ±2200

9000 ±4100

N.D.

Empty vector (pDS12)

2.0 ±0.5

8.4 ±3.5

4 ±0

  1. aPromoters assayed in lacZ-reporter plasmids pDS11 and pDS12: intergenic promoters are predicted promoters for the glnALG, glnKamtB and rtcBA operons; intragenic promoters were identified in the ChIP-chip assay (see Results). Controls are the σ70-dependent, circle-junction promoter from IS492 and the empty vector, pDS12.
  2. bβ-galactosidase assays were performed in MOPS minimal medium (WT and WT+DctD250) or in nitrogen-limiting MOPS (WT-N).
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BMC Genomics

ISSN: 1471-2164

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