Skip to main content
Figure 1 | BMC Genomics

Figure 1

From: Screening of a Brassica napus bacterial artificial chromosome library using highly parallel single nucleotide polymorphism assays

Figure 1

Multidimensional BAC pooling. Two-hundred sixteen 384-well microtiter plates were arranged in a cube consisting of 36 layers of six plates each. The clones were pooled according to their position in the cube along the six distinct coordinate axes. In total, 276 six-dimensional pools were generated. The SA and SB pools were generated according to the scheme shown by combining clones of six and eight plates, respectively. For the screening of all eight dimensions 339 pools had to be analyzed. An additional dimension consisted of 216 pools that contained clones of single 384-well microtiter plates.

Back to article page