Figure 4

Analysis of 19q13 amplicon genes. (A) Heatmap representation of aCGH profiles [6] across the 19q13 region for a set of 70 pancreatic cancer cell lines and early-passage xenografts. Red intensity reflects DNA amplification (see key). Note, the smallest common region of amplification includes 12 genes (indicated) but excludes AKT2 (labeled). Those pancreatic cancer cell lines also analyzed in the shRNA screen are identified by a star; those with 19q13 amplification are identified by a red star. Figure modified from [6]. (B) Heatmap depicts shRNA depletion/enrichment profiles for shRNAs targeting PLEKHG2 and MED29. R and q values are indicated (see Figure 3 legend). Cell lines also included in the aCGH analysis are identified by a star; those with 19q13 amplification by a red star. Note that shRNA depletion levels are highest in pancreatic cell lines with 19q13 amplification.