Figure 6

Effects of disrupting PDHE1β with RNAi on NP and TP locusts. (A) Gene expression of PDHE1α and PDHE1β after RNAi. Data were presented as Mean ± SD (Student’s t-test,*p < 0.05, n = 6). (B) Stupor ratios of NP and TP locusts under 2.0 kPa p O₂ after RNAi. Square roots of stupor ratios were calculated and then were arcsine-transformed to conduct Student’s t-test. Data were presented as Mean ± SD (Student’s t-test,*p < 0.05, n = 6). (C) Energy charge (EC) in flight muscles of NP and TP locusts after RNAi. $\mathit{\text{EC}}=\frac{\left[\mathit{\text{ATP}}\right]+0.5\times \left[\mathit{\text{ADP}}\right]}{\left[\mathit{\text{ATP}}\right]+\left[\mathit{\text{ADP}}\right]+\left[\mathit{\text{AMP}}\right]}$, [ATP], [ADP] and [AMP] represent the concentrations of ATP, ADP and AMP, respectively. Data were presented as Mean ± SD (Student’s t-test,*p < 0.05, n = 6).