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Figure 7 | BMC Genomics

Figure 7

From: Extensively duplicated and transcriptionally active recent lateral gene transfer from a bacterial Wolbachia endosymbiont to its host filarial nematode Brugia malayi

Figure 7

Transcriptional activity of nuwts. (A) Transcription levels of full-length protein-coding nuwts are illustrated with a heatmap of RPKMs on a blue-white-red gradient using publicly available data[22]. Blue represents no expression and red represents the most expression with six stages examined: EE, eggs and embryos; IM, immature microfilariae; MM, mature microfilariae; L3, 3rd stage larvae; L4, 4th stage larvae; AM, adult males; AF, adult females. Wbm0693 (hypothetical protein) is highly transcribed in L3 stage infective larvae, and Wbm0081 and Wbm0783 are highly transcribed in eggs/embryos. (B) Four genes were shown to be constitutively expressed and were subsequently used in experiments to validate the results with Wbm0693: green, Bm1_03960, ribosomal protein L16; purple, Bm1_03910, 40S ribosomal protein S27; blue, actin; red, tpH-1; black, average. (C) Relative abundance of the nuwt originating from Wbm0693 is illustrated relative to the average of the four control genes from panel B (black line). The amplified region of the nuwt contains two SNPs that differentiate the nuwt copy of the gene from the Wolbachia copy allowing the percentage of the clones containing high-quality, nuwt-specific SNPs (blue bars) and the percentage of positions containing high-quality, nuwt-specific SNPs (red bars) to be calculated. The vast majority of the transcripts in the L3, L4, male, and female stages originate from the nuwt copy. (D) The paired end reads generated in this study (gray) and the publicly available RNASeq data (EE, green; IM, light blue, MM, red; L3, magenta; L4, orange; AM, purple; AF, pink) were mapped to the region in the B. malayi genome containing Wbm0693. The region amplified in the qRT-PCR experiments presented in Panels B and C is also illustrated. (E) Genomic features are illustrated for the same region as in Panel D.

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