- Research article
- Open Access
Different patterns of gene structure divergence following gene duplication in Arabidopsis
© Wang et al.; licensee BioMed Central Ltd. 2013
- Received: 2 May 2013
- Accepted: 20 September 2013
- Published: 24 September 2013
Divergence in gene structure following gene duplication is not well understood. Gene duplication can occur via whole-genome duplication (WGD) and single-gene duplications including tandem, proximal and transposed duplications. Different modes of gene duplication may be associated with different types, levels, and patterns of structural divergence.
In Arabidopsis thaliana, we denote levels of structural divergence between duplicated genes by differences in coding-region lengths and average exon lengths, and the number of insertions/deletions (indels) and maximum indel length in their protein sequence alignment. Among recent duplicates of different modes, transposed duplicates diverge most dramatically in gene structure. In transposed duplications, parental loci tend to have longer coding-regions and exons, and smaller numbers of indels and maximum indel lengths than transposed loci, reflecting biased structural changes in transposed duplications. Structural divergence increases with evolutionary time for WGDs, but not transposed duplications, possibly because of biased gene losses following transposed duplications. Structural divergence has heterogeneous relationships with nucleotide substitution rates, but is consistently positively correlated with gene expression divergence. The NBS-LRR gene family shows higher-than-average levels of structural divergence.
Our study suggests that structural divergence between duplicated genes is greatly affected by the mechanisms of gene duplication and may be not proportional to evolutionary time, and that certain gene families are under selection on rapid evolution of gene structure.
- Gene structure
- Transposed duplication
- Whole-genome duplication
Gene duplication is an important mechanism for evolution of functional novelty and increase of genome complexity . Gene duplication may occur by different modes such as whole-genome duplication (WGD)  and single-gene duplications [3–5]. For example, Arabidopsis thaliana has experienced at least three WGD events—two recent events (α and β) since its divergence from other members of the Brassicales clade and a more ancient event (γ) shared with most if not all eudicots . Single-gene duplications including local (tandem or proximal) and dispersed duplications also contribute to the origin of a substantial portion of Arabidopsis genes [5, 7, 8]. Transposed gene duplications, which relocate duplicated genes to new chromosomal positions via either DNA or RNA-based mechanisms [7, 9], may contribute to the widespread existence of dispersed duplicates in the Arabidopsis genome [5, 7].
Since a likely consequence of gene duplication is reversion to single copy (singleton) status , mechanisms for the retention of duplicated genes have been extensively studied. The ‘neo-functionalization’ model suggests that each of two duplicated genes can be retained if at least one evolves modified or novel functions . The ‘sub-functionalization’ model suggests that both duplicated genes can be preserved if they partition the functions of their ancestor, through accumulation of degenerative mutations [10, 11]. More recent models for gene retention include genetic buffering , functional redundancy [13–15], dosage balance constraints [5, 16, 17], or need for enhanced expression levels [18, 19].
Retention of duplicated genes does not occur randomly. Following duplication, genes belonging to some functional categories have been preferentially restored to singleton status across different eukaryotic lineages . In plants, modes of gene duplication retain genes in a biased manner . Genes related to transcription factors, protein kinases, and ribosomal proteins are preferentially retained following WGDs [4, 21], while those genes related to abiotic and biotic stress are more likely to be retained following local duplications [22, 23]. Gene transpositions are more frequent in some families such as F-box, MADS-box, NBS-LRR, and defensins than others [5, 8].
Evolutionary consequences following different modes of gene duplication have been widely investigated. Duplicated genes retained from WGDs show lower levels of expression divergence [24–27], functional innovation [28, 29], network rewiring [29, 30] and epigenetic changes  than single-gene duplicates. Moreover, among single-gene duplications, transposed duplicates tend to evolve faster than tandem or proximal duplicates [25–27, 31].
Functional divergence between duplicated genes was presumed to be driven by nucleotide substitutions including enhancer/promoter mutations, and non-synonymous and synonymous substitutions [24–27]. However, insertions/deletions (indels) between duplicated genes, which may cause shifts of reading frame , have greater effects on the divergence in protein secondary structures [33–35]. In addition, duplicated genes also diverge in exon-intron structures following gene duplication, which was suggested to play an important role during the evolution of duplicated genes . These facts, taken together, suggest that divergence in gene structures such as exon configuration and indels may also drive the functional divergence between duplicated genes.
In this paper, we study structural divergence between duplicated genes in Arabidopsis thaliana. We describe levels of structural divergence between duplicated genes using four different measures. Structural divergence is compared among different modes of gene duplication including WGD, and tandem, proximal and transposed duplications, and then related to duplication epochs, nucleotide substitutions and expression divergence. Evolutionary mechanisms for gene-structure divergence are also investigated.
Comparison of structural divergence among different modes of gene duplication
Transposed duplications are often associated with biased changes in gene structure
Structural divergence and duplication epochs
Structural divergence and nucleotide substitutions
For duplicated genes, structural divergence and nucleotide substitution are two major types of sequence divergence . We compared non-synonymous substitution rates (Ka) among different epochs of gene duplication within WGDs and transposed duplications, and found the following trend: α WGD < β WGD < transposed (<16 Mya) < γ WGD < transposed (16–107 Mya) (comparisons between consecutive gene groups are significant at α = 0.05, Wilcoxon test). However, structural divergence of recent transposed duplications (<16 Mya) tend to be higher (except being measured by numbers of indels) than that of γ WGD (Figure 4), suggesting that gene structure can evolve much faster than nucleotide substitutions.
Correlations between structural divergence and nucleotide substitution rates for all duplicate gene pairs
Measure of structural divergence
Correlation (P-value) with
Difference in coding-region lengths
−0.175 (1.841 × 10-75)
Difference in average exon lengths
Number of indels
Maximum indel length
0.040 (3.316 × 10-5)
0.035 (2.169 × 10-4)
Structural divergence and gene expression divergence
Correlations between structural divergence and gene expression divergence for all duplicate gene pairs
Measure for structural divergence
Difference in coding-region lengths
Difference in average exon lengths
3.46 × 10-11
Number of indels
1.561 × 10-7
Maximum indel length
The NBS-LRR gene family shows higher-than-average structural divergence
Comparison of structural divergence of duplicated genes between the NBS-LRR gene family and all duplicate gene pairs
Measure for structural divergence
NBS-LRR gene family mean
Difference in coding-region lengths
4.984 × 10-3
Difference in average exon lengths
4.434 × 10-3
Number of indels
5.367 × 10-16
Maximum indel length
1.139 × 10-3
Ks increases approximately linearly with time only for relatively low levels of sequence divergence , meaning that there is great uncertainty in using Ks to represent evolutionary time. Thus, to ensure more accurate analyses, we did not use the correlation between structural divergence and Ks to investigate how structural divergence changes over time. Patterns of gene colinearity conservation within and between genomes can be used to estimate the epochs for WGDs and gene transpositions as previously described [6, 40, 41]. After assigning different epochs to gene duplication modes, we used their Ks distributions only for confirming the order of their relative ages.
Classical population genetic theories suggest that duplicated genes have identical sequences immediately following duplication, and then gradually diverge over evolutionary time . The observation that structural divergence between WGD duplicates increases with time is consistent with this classical theory. Due to the fact that most tandem/proximal duplicates are relatively younger than the most recent, Arabidopsis-specific α WGD (Figure 1), comparison between different epochs of tandem/proximal duplications are not feasible in this work. However, the observation that transposed duplications show dramatic and biased structural changes is inconsistent with the classical theory – but consistent with the observation that various types of transposable elements frequently only duplicate gene fragments [37, 38].
The observation that there is a decrease of maximum indel lengths between the transposed duplications that occurred <16 Mya and 16–107 Mya suggests that structural divergence between duplicated genes may not be proportional to evolutionary time. More variations in maximum indel lengths in recently transposed genes could indicate that many transposed duplicates are essentially pseudogenes and not performing important functions , mixed in with the few that confer a striking, adaptive change that may render them finally preserved. However, it should be noted that the striking structural changes that are beneficial still require the intactness of key biological functions, and the transposed genes with extreme structural changes seldom survive over long evolutionary time.
This study reveals that structural divergence between duplicated genes, measured in different ways, shows different patterns depending on modes of gene duplication, and can be affected by both neutral evolution and selection. Changes in gene structure between duplicated genes involve not only alteration of exon-intron structure [36, 42] and gain/loss of introns , but also gain/loss of DNA segments within coding-regions [37, 38] which occurs more extensively in transposed duplications. Certainly there can be more measures to describe structural divergence between duplicated genes, and new biological insights can be generated based on novel measures for structural divergence. For duplicated genes, structural divergence seems more complicated than nucleotide substitutions. Future studies toward better understanding of the evolutionary mechanisms for gene structure changes are necessary.
In this work, we investigated structural divergence between Arabidopsis duplicated genes. We found that transposed duplicates diverge more dramatically in gene structure than genes duplicated by other modes, and that the structural changes in transposed duplications are biased toward shorter length and lower complexity. Structural divergence increases with evolutionary time for WGDs, but not transposed duplications, possibly because genes experiencing severe changes are preferentially lost. Structural divergence between duplicated genes is related to nucleotide substitution rates in different manners, but consistently positively correlated with expression divergence. The NBS-LRR gene family shows higher-than-average levels of structural divergence. This study suggests that structural divergence between duplicated genes, greatly affected by the mechanisms of gene duplication, may be not proportional to evolutionary time, and that certain gene families are under selection on rapid evolution of gene structure.
Genome annotations for Arabidopsis thaliana, Brassica rapa, Populus trichocarpa and Vitis vinifera were obtained from Phytozome v8.0 (http://www.phytozome.net). For genes with multiple transcripts, only the longest transcript was used in related analyses.
Identification of gene duplication modes in Arabidopsis
Transposable element-related genes in Arabidopsis were excluded from analysis. Arabidopsis WGD duplicates were initially obtained from a previous study . Then, α WGD duplicates were updated according to another study , to exclude tandemly-duplicated WGD duplicates which were shown to have very similar evolutionary patterns with tandem duplicates . The WGD duplicate pairs included 3181 α, 1451 β and 521 γ pairs. Other modes of gene duplication were identified from the BLASTP result  of the Arabidopsis thaliana genome (E-value < 10-10 & top five non-self hits for each gene). A total of 2130 tandem and 784 proximal duplications were obtained based on the following criteria: tandem duplications were BLASTP hits to consecutive genes in the genome; proximal duplications were BLASTP hits to nearby genes in the genome interrupted by fewer than ten non-paralogous genes.
To identify Arabidopsis transposed duplications, WGD duplicate pairs and tandem and proximal duplications were removed from the BLASTP result. In Arabidopsis, ancestral loci were the colinear genes between Arabidopsis and its outgroups (related genomes showing colinearity with Arabidopsis), and the non-colinear genes were deemed to be novel loci. Arabidopsis transposed duplications were the BLASTP hits consisting of an ancestral chromosomal locus and a novel locus. Note that based on different sets of outgroups, transposed duplications that occurred within different epochs can be inferred [40, 41]. Using Brassica rapa, Populus trichocarpa and Vitis vinifera as outgroups, we identified 1701 transposed duplications which occurred after Arabidopsis-Brassica divergence, i.e. <16 Million years ago (Mya). Using Populus trichocarpa and Vitis vinifera as outgroups, we identified 2731 transposed duplications which occurred after Arabidopsis-Populus divergence, i.e. <107 Mya. By subtraction of the above two sets of transposed duplications, the remained 1862 transposed duplications were inferred to have occurred between Arabidopsis-Brassica and Arabidopsis-Populus divergence, i.e. 16–107 Mya. Arabidopsis duplicated genes of different modes are listed in Additional file 1.
Indels between duplicated genes
The protein sequences of two duplicated genes were aligned using Clustalw  with default parameters. The Clustalw alignment was then transformed to a “fasta” format alignment, in which, gaps, i.e. consecutive “-”, were deemed to be indels.
Coding sequence divergence
Coding sequence divergence was measured by non-synonymous (Ka) and synonymous (Ks) substitution rates. The protein sequences of duplicate genes were aligned using Clustalw  with default parameters. Then, the protein sequence alignment was converted to a coding sequence alignment using the “Bio::Align::Utilities” module in the BioPerl package (http://www.bioperl.org/). Finally, Ka and Ks were calculated using the Yang & Nielsen method  via the “Bio::Tools::Run::Phylo::PAML::Yn00” module in the BioPerl package.
Gene expression data
Gene expression data generated from the Affymetrix Arabidopsis ATH1 Genome Array (GPL198) were obtained from previous studies [26, 49]. The expression divergence between duplicated genes was measured by 1-r, where r is the Pearson’s correlation coefficient between their expression profiles .
AHP appreciates funding from the National Science Foundation (NSF: DBI 0849896, MCB 0821096, MCB 1021718). YW was supported by an National Science Foundation grant (IOS #1127017) to Dr. Qi Sun at Cornell University. This study was supported in part by resources and technical expertise from the Georgia Advanced Computing Resource Center, a partnership between the Office of the Vice President for Research and the Office of the Chief Information Officer.
- Ohno S: Evolution by gene duplication. 1970, New York: Springer VerlagView ArticleGoogle Scholar
- Paterson AH, Freeling M, Tang H, Wang X: Insights from the comparison of plant genome sequences. Annu Rev Plant Biol. 2010, 61: 349-372. 10.1146/annurev-arplant-042809-112235.View ArticlePubMedGoogle Scholar
- Blanc G, Wolfe KH: Widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes. Plant Cell. 2004, 16 (7): 1667-1678. 10.1105/tpc.021345.PubMed CentralView ArticlePubMedGoogle Scholar
- Maere S, De Bodt S, Raes J, Casneuf T, Van Montagu M, Kuiper M, Van de Peer Y: Modeling gene and genome duplications in eukaryotes. Proc Natl Acad Sci U S A. 2005, 102 (15): 5454-5459. 10.1073/pnas.0501102102.PubMed CentralView ArticlePubMedGoogle Scholar
- Freeling M: Bias in plant gene content following different sorts of duplication: tandem, whole-genome, segmental, or by transposition. Annu Rev Plant Biol. 2009, 60: 433-453. 10.1146/annurev.arplant.043008.092122.View ArticlePubMedGoogle Scholar
- Bowers JE, Chapman BA, Rong J, Paterson AH: Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature. 2003, 422 (6930): 433-438. 10.1038/nature01521.View ArticlePubMedGoogle Scholar
- Wang Y, Wang X, Paterson AH: Genome and gene duplications and gene expression divergence: a view from plants. Ann N Y Acad Sci. 2012, 1256: 1-14. 10.1111/j.1749-6632.2011.06384.x.View ArticlePubMedGoogle Scholar
- Freeling M, Lyons E, Pedersen B, Alam M, Ming R, Lisch D: Many or most genes in Arabidopsis transposed after the origin of the order Brassicales. Genome Res. 2008, 18 (12): 1924-1937. 10.1101/gr.081026.108.PubMed CentralView ArticlePubMedGoogle Scholar
- Cusack BP, Wolfe KH: Not born equal: increased rate asymmetry in relocated and retrotransposed rodent gene duplicates. Mol Biol Evol. 2007, 24 (3): 679-686.View ArticlePubMedGoogle Scholar
- Lynch M, Force A: The probability of duplicate gene preservation by subfunctionalization. Genetics. 2000, 154 (1): 459-473.PubMed CentralPubMedGoogle Scholar
- Lynch M, Conery JS: The evolutionary fate and consequences of duplicate genes. Science. 2000, 290 (5494): 1151-1155. 10.1126/science.290.5494.1151.View ArticlePubMedGoogle Scholar
- Chapman BA, Bowers JE, Feltus FA, Paterson AH: Buffering of crucial functions by paleologous duplicated genes may contribute cyclicality to angiosperm genome duplication. Proc Natl Acad Sci USA. 2006, 103 (8): 2730-2735. 10.1073/pnas.0507782103.PubMed CentralView ArticlePubMedGoogle Scholar
- Dean EJ, Davis JC, Davis RW, Petrov DA: Pervasive and persistent redundancy among duplicated genes in yeast. PLoS Genet. 2008, 4 (7): e1000113-10.1371/journal.pgen.1000113.PubMed CentralView ArticlePubMedGoogle Scholar
- Gu Z, Steinmetz LM, Gu X, Scharfe C, Davis RW, Li WH: Role of duplicate genes in genetic robustness against null mutations. Nature. 2003, 421 (6918): 63-66. 10.1038/nature01198.View ArticlePubMedGoogle Scholar
- Kafri R, Dahan O, Levy J, Pilpel Y: Preferential protection of protein interaction network hubs in yeast: evolved functionality of genetic redundancy. Proc Natl Acad Sci USA. 2008, 105 (4): 1243-1248. 10.1073/pnas.0711043105.PubMed CentralView ArticlePubMedGoogle Scholar
- Freeling M, Thomas BC: Gene-balanced duplications, like tetraploidy, provide predictable drive to increase morphological complexity. Genome Res. 2006, 16 (7): 805-814. 10.1101/gr.3681406.View ArticlePubMedGoogle Scholar
- Birchler JA, Veitia RA: The gene balance hypothesis: from classical genetics to modern genomics. Plant Cell. 2007, 19 (2): 395-402. 10.1105/tpc.106.049338.PubMed CentralView ArticlePubMedGoogle Scholar
- Aury JM, Jaillon O, Duret L, Noel B, Jubin C, Porcel BM, Segurens B, Daubin V, Anthouard V, Aiach N: Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia. Nature. 2006, 444 (7116): 171-178. 10.1038/nature05230.View ArticlePubMedGoogle Scholar
- Bekaert M, Edger PP, Pires JC, Conant GC: Two-phase resolution of polyploidy in the Arabidopsis metabolic network gives rise to relative and absolute dosage constraints. Plant Cell. 2011, 23 (5): 1719-1728. 10.1105/tpc.110.081281.PubMed CentralView ArticlePubMedGoogle Scholar
- Paterson AH, Chapman BA, Kissinger JC, Bowers JE, Feltus FA, Estill JC: Many gene and domain families have convergent fates following independent whole-genome duplication events in Arabidopsis, Oryza, Saccharomyces and Tetraodon. Trends Genet. 2006, 22 (11): 597-602. 10.1016/j.tig.2006.09.003.View ArticlePubMedGoogle Scholar
- Blanc G, Wolfe KH: Functional divergence of duplicated genes formed by polyploidy during Arabidopsis evolution. Plant Cell. 2004, 16 (7): 1679-1691. 10.1105/tpc.021410.PubMed CentralView ArticlePubMedGoogle Scholar
- Rizzon C, Ponger L, Gaut BS: Striking similarities in the genomic distribution of tandemly arrayed genes in Arabidopsis and rice. PLoS Comput Biol. 2006, 2 (9): e115-10.1371/journal.pcbi.0020115.PubMed CentralView ArticlePubMedGoogle Scholar
- Hanada K, Zou C, Lehti-Shiu MD, Shinozaki K, Shiu SH: Importance of lineage-specific expansion of plant tandem duplicates in the adaptive response to environmental stimuli. Plant Physiol. 2008, 148 (2): 993-1003. 10.1104/pp.108.122457.PubMed CentralView ArticlePubMedGoogle Scholar
- Casneuf T, De Bodt S, Raes J, Maere S, Van de Peer Y: Nonrandom divergence of gene expression following gene and genome duplications in the flowering plant Arabidopsis thaliana. Genome Biol. 2006, 7 (2): R13-10.1186/gb-2006-7-2-r13.PubMed CentralView ArticlePubMedGoogle Scholar
- Ganko EW, Meyers BC, Vision TJ: Divergence in expression between duplicated genes in Arabidopsis. Mol Biol Evol. 2007, 24 (10): 2298-2309. 10.1093/molbev/msm158.View ArticlePubMedGoogle Scholar
- Wang Y, Wang X, Tang H, Tan X, Ficklin SP, Feltus FA, Paterson AH: Modes of gene duplication contribute differently to genetic novelty and redundancy, but show parallels across divergent angiosperms. PLoS One. 2011, 6 (12): e28150-10.1371/journal.pone.0028150.PubMed CentralView ArticlePubMedGoogle Scholar
- Li Z, Zhang H, Ge S, Gu X, Gao G, Luo J: Expression pattern divergence of duplicated genes in rice. BMC Bioinformatics. 2009, 6 (10): S8-View ArticleGoogle Scholar
- Hakes L, Pinney JW, Lovell SC, Oliver SG, Robertson DL: All duplicates are not equal: the difference between small-scale and genome duplication. Genome Biol. 2007, 8 (10): R209-10.1186/gb-2007-8-10-r209.PubMed CentralView ArticlePubMedGoogle Scholar
- Guan Y, Dunham MJ, Troyanskaya OG: Functional analysis of gene duplications in Saccharomyces cerevisiae. Genetics. 2007, 175 (2): 933-943. 10.1534/genetics.106.064329.PubMed CentralView ArticlePubMedGoogle Scholar
- Arabidopsis Interactome Mapping Consortium: Evidence for network evolution in an Arabidopsis interactome map. Science. 2011, 333 (6042): 601-607.PubMed CentralView ArticleGoogle Scholar
- Wang Y, Wang X, Lee TH, Mansoor S, Paterson AH: Gene body methylation shows distinct patterns associated with different gene origins and duplication modes and has a heterogeneous relationship with gene expression in Oryza sativa (rice). New Phytol. 2013, 198 (1): 274-283. 10.1111/nph.12137.View ArticlePubMedGoogle Scholar
- Raes J, Van de Peer Y: Functional divergence of proteins through frameshift mutations. Trends Genet. 2005, 21 (8): 428-431. 10.1016/j.tig.2005.05.013.View ArticlePubMedGoogle Scholar
- Guo B, Zou M, Wagner A: Pervasive indels and their evolutionary dynamics after the fish-specific genome duplication. Mol Biol Evol. 2012, 29 (10): 3005-3022. 10.1093/molbev/mss108.View ArticlePubMedGoogle Scholar
- Zhang Z, Huang J, Wang Z, Wang L, Gao P: Impact of indels on the flanking regions in structural domains. Mol Biol Evol. 2011, 28 (1): 291-301. 10.1093/molbev/msq196.View ArticlePubMedGoogle Scholar
- Zhang Z, Wang Y, Wang L, Gao P: The combined effects of amino acid substitutions and indels on the evolution of structure within protein families. PLoS One. 2010, 5 (12): e14316-10.1371/journal.pone.0014316.PubMed CentralView ArticlePubMedGoogle Scholar
- Xu G, Guo C, Shan H, Kong H: Divergence of duplicate genes in exon-intron structure. Proc Natl Acad Sci USA. 2012, 109 (4): 1187-1192. 10.1073/pnas.1109047109.PubMed CentralView ArticlePubMedGoogle Scholar
- Juretic N, Hoen DR, Huynh ML, Harrison PM, Bureau TE: The evolutionary fate of MULE-mediated duplications of host gene fragments in rice. Genome Res. 2005, 15 (9): 1292-1297. 10.1101/gr.4064205.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang YE, Vibranovski MD, Krinsky BH, Long M: A cautionary note for retrocopy identification: DNA-based duplication of intron-containing genes significantly contributes to the origination of single exon genes. Bioinformatics. 2011, 27 (13): 1749-1753. 10.1093/bioinformatics/btr280.PubMed CentralView ArticlePubMedGoogle Scholar
- Li WH: Molecular Evolution. 1997, Sunderland, Massachusetts: Sinauer AssociatesGoogle Scholar
- Woodhouse MR, Tang H, Freeling M: Different gene families in Arabidopsis thaliana transposed in different epochs and at different frequencies throughout the rosids. Plant Cell. 2011, 23 (12): 4241-4253. 10.1105/tpc.111.093567.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang Y, Li J, Paterson AH: MCScanX-transposed: detecting transposed gene duplications based on multiple colinearity scans. Bioinformatics. 2013, 29 (11): 1458-1460. 10.1093/bioinformatics/btt150.View ArticlePubMedGoogle Scholar
- Zhang Z, Zhou L, Wang P, Liu Y, Chen X, Hu L, Kong X: Divergence of exonic splicing elements after gene duplication and the impact on gene structures. Genome Biol. 2009, 10 (11): R120-10.1186/gb-2009-10-11-r120.PubMed CentralView ArticlePubMedGoogle Scholar
- Knowles DG, McLysaght A: High rate of recent intron gain and loss in simultaneously duplicated Arabidopsis genes. Mol Biol Evol. 2006, 23 (8): 1548-1557. 10.1093/molbev/msl017.View ArticlePubMedGoogle Scholar
- Thomas BC, Pedersen B, Freeling M: Following tetraploidy in an Arabidopsis ancestor, genes were removed preferentially from one homeolog leaving clusters enriched in dose-sensitive genes. Genome Res. 2006, 16 (7): 934-946. 10.1101/gr.4708406.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang Y: Locally duplicated ohnologs evolve faster than nonlocally duplicated ohnologs in Arabidopsis and rice. Genome Biol Evol. 2013, 5 (2): 362-369. 10.1093/gbe/evt016.PubMed CentralView ArticlePubMedGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215 (3): 403-410.View ArticlePubMedGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22 (22): 4673-4680. 10.1093/nar/22.22.4673.PubMed CentralView ArticlePubMedGoogle Scholar
- Yang Z, Nielsen R: Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models. Mol Biol Evol. 2000, 17 (1): 32-43. 10.1093/oxfordjournals.molbev.a026236.View ArticlePubMedGoogle Scholar
- Spangler JB, Subramaniam S, Freeling M, Feltus FA: Evidence of function for conserved noncoding sequences in Arabidopsis thaliana. New Phytol. 2012, 193 (1): 241-252. 10.1111/j.1469-8137.2011.03916.x.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.