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Figure 3 | BMC Genomics

Figure 3

From: Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains

Figure 3

Identification of DNA binding motifs for the A, B and C zinc finger domains of FruM. (A) Consensus DNA binding sequences of the zinc finger DNA binding domains (DBD) of FruMA, FruMB or FruMC identified after ten rounds of SELEX. (B) Oligonucleotides containing the consensus sequences identified by SELEX, or sequences of the same nucleotide content but randomized in order, for DBDA or DBDC were incubated with GST-FruA DBD or GST-FruC DBD, or GST alone and assayed by gel mobility shift assay. GST-FruA DBD and GST-FruC DBD bound to oligonucleotides containing their respective consensus sequence (Lane 2 and 8) have slower mobility than GST (Lane 4 and 10), GST-FruA DBD (Lane 3 and 12), or GST-FruC DBD (Lane 6 and 9) incubated with oligonucleotides containing random sequences or the consensus sequence from other zinc finger domains or free oligonucleotides (no protein added in Lanes 1, 5, 7,11).

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