Tpk2 promoter binding during hyphal induction. Tpk2 localization was determined in cells induced for hypha formation at SOK1 (A), HYR1 (B) and ECE1 (C) genomic loci. ChIP chip occupancy values of Tpk2HA were determined in duplicates as in Figure 4; peak binding areas are marked by a dotted box. All three genes contain Efg1 binding sites within their promoters (circles) at the indicated coordinates; positions marked were reported by Nobile et al. and by Lu et al. (asterisks). The kinked arrow indicates the SOK1 transcript start site . Transcript levels for each gene during yeast growth or hyphal induction were determined by qPCR as in Figure 4B.