Skip to main content
Figure 2 | BMC Genomics

Figure 2

From: Transcriptome analysis of the filamentous fungus Aspergillus nidulans directed to the global identification of promoters

Figure 2

Antisense transcription at the meaB locus. (A) Antisense transcription was identified overlapping the 5′ end of the meaB gene. Coverage on the positive strand for 5′-specific and whole transcriptome RNA-seq libraries is shown in the top two plots. Coverage on the negative strand for these libraries is shown in the bottom two plots. The meaB gene (AN4900) is encoded on the negative strand (blue boxes and thin black lines represent exons and introns/UTR, blue dotted lines indicate multiple transcription start sites). Red boxes indicate predicted antisense transcripts, their transcription start sites shown by red dotted lines. (B) From northern analysis of the wild type (WT) the antisense transcript (meaB-AS) was identified and found to be modulated by nitrogen regime; NH4+, glutamine (Gln), alanine (Ala) as sole nitrogen source or nitrogen starvation (-N), for two hours. Analysis of a strain bearing a loss of function mutation in areA (areA49) revealed that meaB-AS expression is AreA dependent. 18S rRNA was used as a control. (C) Quantitative northern analysis of the meaB sense transcript (meaB-S) in the wild type (WT), areA49, meaBΔintron and ΔRNAi strains was undertaken. The mean intensity, relative to WT, of four independent experiments is plotted (+/- SD). Downregulation of meaB transcript levels in response to poor nitrogen (Ala) was lost in the meaBΔintron strain but the response to nitrogen starvation was retained.

Back to article page