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Figure 4 | BMC Genomics

Figure 4

From: The Clostridium small RNome that responds to stress: the paradigm and importance of toxic metabolite stress in C. acetobutylicum

Figure 4

Validation of sRNA expression by Northern analysis. (A) 6S RNA; (B) tmRNA; &(C) SAM. The right arrow ( →) and left arrows (←) indicate the positive or negative orientation of the genes adjacent to the sRNA, while the orientation of the sRNA is represented by the double lined arrow. The symbol P, upstream of the sRNA represents the presence of a promoter region corresponding to one of the sigma factors (A, G, E & F) and the Ϙ symbol at the 3’ downstream end of the sRNA represents the rho independent terminator. The 6S RNA secondary structure shows the conserved asymmetric bubbles with G-C pairs at the end (arrows) (see text for details). BuOH- butanol, BA- butyrate, NS- no stress, 30 & 60 min post stress.

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