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Figure 3 | BMC Genomics

Figure 3

From: A platform independent RNA-Seq protocol for the detection of transcriptome complexity

Figure 3

Comparison of the qRT-PCR and RNA-Seq data. Log2 fold changes calculated with both quantitative Real Time (qRT-PCR) (black) and RNA-seq (light grey for 454 and dark grey for Miseq) analysis are reported. Positive log2 fold changes indicate over-expression in OST-78, while negative values over-expression in OST-83. Five differentially expressed RC nuclear-encoded genes, representing four of the five respiratory complexes (I, III, V, IV) were chosen for the validation in qRT-PCR, together with MT-ND5, one of the mitochondrial-encoded genes found differentially expressed with both the two ;RNA-seq technologies.

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