Figure 6

Mosquito proteins of the W-rich peptides/16-kDa protein family of Culex. (A) Clustal alignment. Symbols above the alignment indicate (*) identical sites, (:) conserved sites, and (.) less-conserved sites. (B) Bootstrapped phylogram derived from the alignment in A. Numbers on the tree bifurcations indicate the percentage bootstrap support above 50%. The scale bar at the bottom represents 20% amino acid substitution. Sequences were aligned by the ClustalW program, and the dendrogram was made with the Mega package after 1,000 bootstraps with the neighbor-joining algorithm. The Psorophora albipes proteins are recognized by the prefix Psor- and a red marker (red circle symbol). Aedes proteins are indicated with a blue marker (blue circle symbol). Other proteins are represented by the first three letters of the genus name, followed by the first three letters of the species name, followed by the gi| accession number. Roman numerals indicate individual clades with strong bootstrap support.