Table 5 List of primers designed for the 19 candidate reference genes considered in this study
Genoscope ID | Gene abbreviation | GenBank accession | Primer sequence (5′-3′) | Product size (bp) | TM (°C) |
|---|---|---|---|---|---|
GSVIVT01038268001 | VvRABI | XM_002280570 | F: GCAAGGCTCAGTGCTGTTTA | 217 | 60 |
R: TTGGGATTGGGTGGCTCATA | |||||
GSVIVT01030215001 | VvPP1 | XM_002268545 | F: GAGCCAGGAATCCACAAAGAC | 166 | 62 |
R: AGAACCGACCAAACCCAAACT | |||||
GSVIVT01016593001 | VvADF2 | XM_002284004 | F: GGCCTTTGTCGCTGTTTCCT | 268 | 60 |
R: AGTGGGCTCACCAACCTTTT | |||||
GSVIVT01028520001 | VvPR26S | XM_002263298 | F: GAGCAAGTTGAAGCCGCAGGAG | 138 | 62 |
R: CCCACGGACGACGACACGAT | |||||
GSVIVT01008708001 | VvTCPB | XM_002285876 | F: AGACAGTGATTGACAGCCGAGTT | 238 | 64 |
R: ATCCCTGCGTGGCTTTCTTCC | |||||
GSVIVT01033771001 | VvSFU2 | XM_002277409 | F: CCCCACCCTCCTCCTTTCCAAC | 192 | 64 |
R: TGGTCAGCCAAATTGTCACAGA | |||||
GSVIVT01028854001 | VvPR40 | XM_002273250 | F: GATTGTGCCTGCCACCTTGA | 257 | 62 |
R: AACCTCCACCTCCTCGTCCA | |||||
GSVIVT01036166001 | VvSAP4 | XM_002262726 | F: AGCCTAATGTGAAGTGGAGC | 179 | 60 |
R: AACAGCCTTGGCTAGGTATG | |||||
GSVIVT01035814001 | VvUNP2 | XM_002284964 | F: AGATACAGAGGCAGGAGAAGT | 214 | 64 |
R: AGAATTGGGAATCCAGTGAGG | |||||
GSVIVT01033172001 | VvSF7 | XM_002272621 | F: GAGCGAGAACTTGAAGATGAG | 258 | 62 |
R: CAAACGGCATTCACGGGCAAA | |||||
GSVIVT01037814001 | VvUNP3 | FQ387200 | F: ACGCTCCTCAGTACGGTCAG | 91 | 60 |
R: AGAGCAGCCAAACATCCTTC | |||||
GSVIVT01016731001 | VvPSA | XM_002271893 | F: ATGGACCTCGCCTCTTCAAAT | 262 | 62 |
R: TCCTCGGTGGACAACACTCTG | |||||
GSVIVT01031067001 | VvTCPZ | XM_002283474 | F: CTTATGAAACAATCAGAACGCTAC | 140 | 62 |
R: TCAGGCTCATCACCCATTACCA | |||||
GSVIVT01025947001 | VvAIG1 | XM_002281960 | F: GAAGATTATTTGGGCCGTGAG | 108 | 62 |
R: CTTCTTGGCTTCATCCTTGGT | |||||
GSVIVT01033442001 | VvCCRP | XM_002280954 | F: TTGGTTTGAAGTTGGACGCTCTA | 173 | 64 |
R: AGTGACGAGGAGTAGGTGAGG | |||||
GSVIVT01027659001 | VvUNP | XM_002280576 | F: TCGGACCTTCGGATTAGCAT | 227 | 60 |
R: CACTCCAGTGGGTAGCATAG | |||||
GSVIVT01015062001 | VvADH7 | XM_002278057 | F: TCCGGCGAATCCTGGATGTTA | 104 | 64 |
R: CCGTCACCACCGCAATCCTCT | |||||
GSVIVT01013003001 | VvPRN26S | XM_003631440 | F: GAAGCTCTGGCACCAACTCACT | 158 | 64 |
R: ACTGCCTAGAAACTATGACAGCAA | |||||
GSVIVT01012792001 | VvSLP | FQ388031 | F: GCCGTCCACATCATTTACACT | 108 | 62 |
R: AGCCTTCTTGGCAGCCTCCTC |