Figure 6
From: A high-throughput Sanger strategy for human mitochondrial genome sequencing
Sequence coverage. Percentage of the codR or full mtGenome with redundant sequence coverage following a single pass of automated data generation for eleven population samples, representing a range of mtDNA haplogroups, processed in duplicate. One sample, for which all sequence data in a single direction for a single amplicon was unusable and sourced to instrument failure, was removed from the analysis as an outlier; and small regions of unidirectional sequence coverage due to length heteroplasmy in hypervariable regions one and two in some samples were ignored. On average across the twenty-two samples, high-quality forward and reverse coverage was produced for 99% of the mtGenome.