A high-throughput Sanger strategy for human mitochondrial genome sequencing

BMC Genomics

Table 1 Amplification primers

Amplicon number	Amplicon size	Primer name	Primer sequence	Source
1	2417	F402	ATCTTTTGGCGGTATGCACTTT	New
1	2417	R2818	GCCCCAACCGAAATTTTTAAT	New
2	2381	F2480	AAATCTTACCCCGCCTGTTT	New
2	2381	R4860	GAAGAAGCAGGCCGGATGT	New
3	2291	F4609	AAATAAACCCTCGTTCCACAGA	New
3	2291	R6899	CATATTGCTTCCGTGGAGTGTG	New
4	2511	F6636	ATTCTTATCCTACCAGGCTTCG	New
4	2511	R9146	GCGACAGCGATTTCTAGGATAG	New
5	2489	F8940	CCCCATACTAGTTATTATCGAAACC	New
5	2489	R11428	GGCTTCGACATGGGCTTT	New
6	2759	F11319	CAAACTCCTGAGCCAACAACTT	New
6	2759	R14077	TTTGGGTTGAGGTGATGATG	New
7	2208	F13835	CAGCCCTAGACCTCAACTACC	New
7	2208	R16042	CTGCTTCCCCATGAAAGAAC	[19]
8	1198	F15971	TTAACTCCACCATTAGCACC	[13]
8	1198	R599	TTGAGGAGGTAAGCTACATA	[13]

Amplification primer sequences for the eight mtGenome amplicons. The primers for Amplicon 8, which covers the mtDNA CR, were adopted from [13]. The reverse primer for Amplicon 7 (R16042) was previously designed for use as a sequencing primer [19]. All primers except F2480 (Amplicon 2), R4860 (Amplicon 2), R9146 (Amplicon 4) and R14077 (Amplicon 6) are also used for sequencing (see Table 2).

ISSN: 1471-2164