A BAC based physical map and genome survey of the rice false smut fungus Villosiclava virens

BMC Genomics

Table 1 Statistics of the BAC Library, fingerprints and BESs of V. virens strain UV-8b

Category	Value
Clone number	5760
Empty rate	< 0.1%
Average insert size	140 kb
Genome coverage¹	22.7X
Clones end-sequenced	4512
Clones with BESs	3722
Paired-end BESs	2838
Single-end BESs	884
Average BES length	462 bp
GC content	51.52%
Clones fingerprinted	2688
Clones with fingerprints data²	2290
Assembled into FPC contigs	2035
With paired-end BESs	1557
With single-end BESs	407
As singletons	255
With paired-end BESs	194
With single-end BESs	48
Average bands per clone³	124

¹The genome size of V. virens was estimated as 35.5 Mb.
²The clones that contained 50–200 bands were imported into FPC for contig assembly.
³The total bands of all successfully fingerprinted clones were 283391.

ISSN: 1471-2164