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Table 2 Summary of the UV-8b physical maps autobuilt from assembly of the 2,290 BAC clones at different stringencies

From: A BAC based physical map and genome survey of the rice false smut fungus Villosiclava virens

Cutoff

Contigs

Avr contig length (kb)1

Longest contig (kb)1

Physical length (Mb)1

Q contigs/ Q clones (%)2

No. contigs containing different clone numbers

Singletons (%)3

>50

50-26

25-10

9-3

=2

10-30

314

184

287

58.2

0/0 (0.00)

0

0

18

180

116

991 (43.3)

10-25

305

196

376

60.0

7/7 (0.31)

0

0

43

168

94

653 (28.5)

10-20

255

217

774

55.5

36/49 (2.14)

0

4

57

136

58

428 (18.7)

10-18

231

230

774

53.4

41/63 (2.75)

0

9

61

112

49

357 (15.6)

10-15

196

261

774

51.3

63/111 (4.85)

1

15

61

78

41

255 (11.1)

10-12

161

302

1032

48.7

74/164 (7.16)

3

20

53

62

23

177 (7.7)

10-10

142

335

1585

47.7

77/174 (7.60)

5

20

51

48

18

160 (7.0)

10-08

135

340

1585

46.0

80/204 (8.91)

5

22

50

44

14

134 (5.9)

Phase IA 4

194

263

774

51.2

63/111 (4.85)

1

15

61

76

41

255 (11.1)

  1. 1Each fingerprint band was estimated to be 1.13 kb based on the average insert size 140 kb and an average 124 bands per clone.
  2. 2The percentage of Q clones to total clones 2290.
  3. 3The percentage of single clones to total clones 2290.
  4. 4The cutoff value of 10-15 was chosen, then contig101was end-merged to contig76, and contig193 was end-merged to contig1 by “End to End” function at cutoff 10-12, respectively.