Table 5 SSR loci amplified from 23 accessions of tree peony
From: Rapid microsatellite development for tree peony and its implications
Locus | 23 accessions | |||||||
|---|---|---|---|---|---|---|---|---|
Repeat | Forward primer (5′-3′) | Reverse primer (5′-3′) | Size (bp) | Ta (ºC) | Na | He | Ho | |
2A | TGG6 | AACTGCGCTAGTCGTCCCCATAAAC | AAAGCCGCCTACAGAGGATGTTCAT | 268 | 57 | 3 | 0.4647 | 0.0435 |
19A | CA16 | TAACATCTCACTACCACTCAGGCGA | CATAAGGGTGATGATCATGTGGTTG | 164 | 54.5 | 3 | 0.6493 | 0.0000 |
25A | TGT10 | CAATCCCTTTTGTAATGCCCCTTTC | CAGGCTGTACTAGCAAAGGCTTCCA | 215 | 54.5 | 3 | 0.5565 | 0.0000 |
26A | TTG7 | TGGGCCCTACAAGTGATGATATTCC | ATGGAATCCAGGTTTGTGAATGTGA | 245 | 54.5 | 3 | 0.559 | 0.0000 |
30A | CA13 | TGTCATACCGACTTCGGCTAGGCTA | AAGGGTGATCGTGTGGTTGATGTTT | 265 | 54.5 | 4 | 0.7275 | 1.0000 |
31A | CT11 | AGCGCGTTTAATTGCTCTTACCTTG | CTCCCTCCTCTAACTCCATGCTTGC | 303 | 54.5 | 3 | 0.6261 | 0.0000 |
36A | (TGG)6gctttggccggttcg(CTT)5 | GACTGTAGTGATGGTGGTGGATTGG | AGCTTATGAACCCTGATGATGACGC | 261 | 57 | 3 | 0.5797 | 0.0000 |
48A | CAG5 | ACAGCGTCAGCAGACAGGAAGTACC | AAGAGTACCTGTCACCCCATCCAAA | 364 | 57 | 4 | 0.5913 | 0.0000 |
49A | TGC5 | TCTGGGTGATAGGTGGAGCTGGTGC | GGAAGACGCCCACAATGAAATCACA | 314 | 57 | 4 | 0.6696 | 0.0000 |
50A | CA13 | CACGGCTTTAAAATGCGTCTCAACT | AGGCTGGTGATAGTGTTGTTGATGC | 252 | 54.5 | 4 | 0.5295 | 0.0000 |
53A | TCC5 | CTCTTGTCAACCCCCACTGCCTCCT | GAAGGGACTTTCGCTGGAATCTGGC | 353 | 59 | 4 | 0.6802 | 0.0000 |
54A | (CT)9(CA)14 | TGTCGGGCGGTAAGTTTAGGGAAGA | CCACTTGGGTTCTGTTGGAGACTCG | 388 | 59 | 3 | 0.5034 | 0.0435 |
56A | AC15 | CAGGTGGCATTTTTGGCTTCTCTCT | TTGGCCCAATCACATGTAATCCCTC | 388 | 57 | 3 | 0.5217 | 0.0000 |
58A | GCA6 | TAGGATGACAAAGTGCAGGAAACCC | TGCTCAAACTCATCCTCAAGCTGTG | 318 | 57 | 2 | 0.085 | 0.0000 |
59A | AC18 | TACAACACTTCTCGCCTAACGCACC | AGACATGGTGCAAGTATGGGAGACG | 270 | 59 | 3 | 0.4908 | 0.0000 |
63A | (TC)9(AC)17 | CACCGCATATCTCCAACCTCACCTC | TTGGGTAGAGATAGGAGGTTGGGGC | 277 | 59 | 3 | 0.6609 | 0.0000 |
65A | TGG5 | CATACCTCCATCATGATGCTGCTGT | ATGAAGGCTCAGTAAGAACCTCGGA | 355 | 57 | 3 | 0.3053 | 0.0000 |
73A | CAG5 | CCATCTCAGGGTCAGGGTTCTCGTA | TAGAGTGTACCTTCACCCCCATCGG | 375 | 59 | 4 | 0.6928 | 0.1379 |
78A | AC16 | TATCAAATGGGGATGGTCTCCTCTT | AATTCTGCCACTATGAGCTCGATCT | 314 | 54.5 | 5 | 0.6899 | 0.1579 |
79A | GCA5 | AGAGGAAGTTTGAGGCCATCAGTCG | CAACTGTAGCCTTCTGTTCCTGCCC | 367 | 57 | 2 | 0.4638 | 0.0000 |
80A | GTG5 | AAGGTTATGGTGGCAGTGAAGATGA | ACCGTCGTACTACCACTTACAGCCG | 207 | 54.5 | 4 | 0.6773 | 0.3043 |
87A | TG15 | TGTAATCGATCGAGTTTCTTGGGTC | CCTAACACTCCACCACTAAGTCGCT | 188 | 56 | 3 | 0.6261 | 0.0000 |
91A | (GT)9ttgta(TG)16 | TCAGCCCCTAGCATAGAAGAATCCA | TCTCACTACCACCTACGCGATGTTC | 384 | 60 | 3 | 0.6032 | 0.0000 |